Abstract
Development of appropriate resin linker combinations for solid-phase peptide synthesis (SPPS) has allowed rapid access to (fully) protected peptide fragments with a free C-teminal carboxyl moiety. These fragments may be assembled either in solution or on resin—an approach that has some intrinsic advantages compared to the stepwise methodology (see Chapter 15). Approaches to synthesize such fragments usually employ “orthogonal” (see Note 1) Nα-protection/side-chain protection. The orthogonal combination Fmoc/tBu can, however, only be used if the peptide resin bond is cleavable either by acids weak enough to leave tBu groups intact or by a method employing neither acids nor bases, such as photolysis (1) or catalysis by a Pd(0) complex (2).
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© 1994 Humana Press Inc.
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Mergler, M. (1994). Synthesis of Fully Protected Peptide Fragments. In: Pennington, M.W., Dunn, B.M. (eds) Peptide Synthesis Protocols. Methods in Molecular Biology, vol 35. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-273-6:287
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DOI: https://doi.org/10.1385/0-89603-273-6:287
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