Abstract
Several immunological procedures can be successfully carried out using nonpurified antibodies, such as unfractionated antisera or ascitic fluid/culture supernatant containing monoclonal antibodies (MAbs). However, a much “cleaner” result can often be obtained if some form of enrichment or isolation of immunoglobulin is employed. Some procedures, such as conjugation with isotopes, fluorochromes, or enzymes, and preparation of immunoaffinity columns cannot usually be efficiently performed with nonpurified immunoglobulin, and some procedures may yield artifactual results if whole antiserum or ascitic fluid is used as a source of antibody. Purification of immunoglobulins is therefore essential or at least useful for a range of immunological methods. This process may consist of purification of total IgG or subpopulations (e.g., subclasses) of IgG from antisera/ascitic fluid/culture supernatant or the isolation of a particular antigen-binding fraction from such fluids. The former can be achieved by biochemical procedures, whereas the latter usually requires some form of affinity purification.
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References
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© 1994 Humana Press Inc., Totowa, NJ
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Page, M., Baines, M.G., Thorpe, R. (1994). Preparation of Purified Immunoglobulin G (IgG). In: Walker, J.M. (eds) Basic Protein and Peptide Protocols. Methods in Molecular Biology™, vol 32. Humana Press. https://doi.org/10.1385/0-89603-268-X:407
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DOI: https://doi.org/10.1385/0-89603-268-X:407
Publisher Name: Humana Press
Print ISBN: 978-0-89603-268-2
Online ISBN: 978-1-59259-519-8
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