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C-Terminal Sequencing of Peptides

The Thiocyanate Degradation Method

  • Protocol
Basic Protein and Peptide Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 32))

Abstract

The possibility of sequencing peptides or proteins from their N-terminal ends became a reality with the discovery and the application of the Edman stepwise degradation (1,2; also see Chapter 36). The procedure developed was automated in 1967 (2), and it is now possible to obtain unequivocal sequence information for up to 35–70 residues for a wide variety of peptides and proteins before the buildup of background material makes assignments difficult, if not impossible. In practice, the complete sequence often has to be derived by establishing the sequences of smaller fragments (i.e., peptides) obtained from the peptide by the action of either enzymes or chemical reagents (3).

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Author information

Authors and Affiliations

  1. EMBL, Heidelberg, Germany

    Franca Casagranda

  2. CSIRO Division of Biomolecular Engineering, Parkville, Australia

    John F. K. Wilshire

Authors
  1. Franca Casagranda
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  2. John F. K. Wilshire
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Editor information

Editors and Affiliations

  1. University of Hertfordshire, Hatfield, UK

    John M. Walker

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© 1994 Humana Press Inc., Totowa, NJ

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Casagranda, F., Wilshire, J.F.K. (1994). C-Terminal Sequencing of Peptides. In: Walker, J.M. (eds) Basic Protein and Peptide Protocols. Methods in Molecular Biology™, vol 32. Humana Press. https://doi.org/10.1385/0-89603-268-X:335

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  • DOI: https://doi.org/10.1385/0-89603-268-X:335

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-268-2

  • Online ISBN: 978-1-59259-519-8

  • eBook Packages: Springer Protocols

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