Abstract
The method described in this chapter is based on derivatization of amino acids, produced by hydrolysis of peptides or proteins, with phenylisothiocyanate. This forms phenylthiocarbamyl amino acids, which are then separated by reversed-phase high-performance liquid chromatography and quantified from their UV absorbance at 254 nm.
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© 1994 Humana Press Inc., Totowa, NJ
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Irvine, G.B. (1994). Amino Acid Analysis. In: Walker, J.M. (eds) Basic Protein and Peptide Protocols. Methods in Molecular Biology™, vol 32. Humana Press. https://doi.org/10.1385/0-89603-268-X:257
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DOI: https://doi.org/10.1385/0-89603-268-X:257
Publisher Name: Humana Press
Print ISBN: 978-0-89603-268-2
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