The Lowry Method for Protein Quantitation

  • Jaap H. Waterborg
  • Harry R. Matthews
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 32)

Abstract

The most accurate method of determining protein concentration is probably acid hydrolysis followed by amino acid analysis. Most other methods are sensitive to the amino acid composition of the protein, and absolute concentrations cannot be obtained. The procedure of Lowry et al. (1) is no exception, but its sensitivity is moderately constant from protein to protein, and it has been so widely used that Lowry protein estimations are a completely acceptable alternative to a rigorous absolute determination in almost all circumstances where protein mixtures or crude extracts are involved.

Keywords

Sugar Vortex 5H2O Hydrolysis Sucrose 

References

  1. 1.
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Copyright information

© Humana Press Inc., Totowa, NJ 1994

Authors and Affiliations

  • Jaap H. Waterborg
    • 1
  • Harry R. Matthews
    • 2
  1. 1.Division of Cell Biology and BiophysicsUniversity of MissouriKansas City
  2. 2.Department of Biological ChemistryUniversity of CaliforniaDavis

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