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The Lowry Method for Protein Quantitation

  • Jaap H. Waterborg
  • Harry R. Matthews
Part of the Methods in Molecular Biology™ book series (MIMB, volume 32)

Abstract

The most accurate method of determining protein concentration is probably acid hydrolysis followed by amino acid analysis. Most other methods are sensitive to the amino acid composition of the protein, and absolute concentrations cannot be obtained. The procedure of Lowry et al. (1) is no exception, but its sensitivity is moderately constant from protein to protein, and it has been so widely used that Lowry protein estimations are a completely acceptable alternative to a rigorous absolute determination in almost all circumstances where protein mixtures or crude extracts are involved.

Keywords

Amino Acid Composition Aromatic Amino Acid Amino Acid Analysis Protein Mixture Acceptable Alternative 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265–275.PubMedGoogle Scholar
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    Waterborg, J. H. and Matthews, H. R. (1984) The Burton Assay for DNA, in Methods in Molecular Biology, vol. 2: Nucleic Acids (Walker, J. M., ed.), Humana, Totowa, NJ, pp. 1–3.Google Scholar
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    Peterson, G. L. (1983) Determination of total protein. Methods Enzymol. 91, 95–121.PubMedCrossRefGoogle Scholar
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    Markwell, M. A. K., Haas, S. M., Tolbert, N. E., and Bieber, L. L. (1981) Protein determination in membrane and lipoprotein samples. Methods Enzymol. 72, 296–303.PubMedCrossRefGoogle Scholar
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    Hess, H. H., Lees, M. B., and Derr, J. E. (1978) A linear Lowry-Folin assay for both water-soluble and sodium dodecyl sulfate-solubilized proteins. Anal. Biochem. 85, 295–300.PubMedCrossRefGoogle Scholar
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    Larson, E., Howlett, B., and Jagendorf, A. (1986) Artificial reductant enhancement of the Lowry method for protein determination. Anal. Biochem. 155, 243–248.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 1994

Authors and Affiliations

  • Jaap H. Waterborg
    • 1
  • Harry R. Matthews
    • 2
  1. 1.Division of Cell Biology and BiophysicsUniversity of MissouriKansas City
  2. 2.Department of Biological ChemistryUniversity of CaliforniaDavis

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