Abstract
Unlike gene expression in prokaryotic cells, which is primarily under transcriptional control, gene expression in eukaryotic cells is subject to both transcriptional and posttranscriptional controls. Since transcription and translation in eukaryotic cells are separated topographically, the regulation of mRNA metabolism can occur at multiple sites, within nuclei and in the cytoplasm. Nevertheless, transcription remains a critical locus of control of eukaryotic gene expression. Transcriptional regulation affects cellular mRNA abundance by affecting rates of tran- scription. Another important control mechanism that can affect mRNA abundance is the rate of mRNA decay. Thus, half-life of mRNA repre- sents a balance between rates of transcription and intracellular degrada- tion, e.g., an increase in the abundance of mRNA could result from decreased mRNA degradation, increased mRNA synthesis, or both.
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References
Sorci-Thomas, M., Wilson, M. D., Johnson, F. L., Williams, D. L., and Rudel, L. L. (1989) Studies on the expression of genes encoding apolipoproteins B-100 and B-48 and the low densiy lipoprotein receptor in non-human primates. Comparison of dietary fat and cholesterol. J. Biol Chem 264, 9039–9045.
Elshourbagy, N. A., Boguski, M. S., Liao, W. S. L., Jefferson, L. S., Gordon, J. I., and Taylor, J. M. (1985) Expression of rat apolipoprotein A-IV and A-I genes: mRNA induction during development and in response to glucocorti-coids and insulin. Proc. Natl. Acad. Sci. USA 82, 8242–8246.
Karathanasis, S. K. and Yunis, S. (1986) Structure, evolution, and tissue-spe-cific synthesis of human apolipoprotein AIV. Biochemistry, 25, 3962–3970
Feng, B., Hilt, D. C., and Max, S. R. (1990) Transcriptional regulation of glutamine synthase gene expression by dexamethasone in L6 muscle cells J. Biol.Chem. 265, 18702–18706.
Brock, M. L. and Shapiro, D. J. (1983) Estrogen regulates the absolute rate of tran-scription of the Xenopus laevis vitellogenin genes. J Biol. Chem. 258, 5449–5455.
McKnight, G. S. and Palmiter, R. D. (1979) Transcriptional regulation of the ovalbumin and conalbumin genes by steroid hormones in chick oviduct. J Biol. Chem. 254, 9050–9058
Chazenbalk, G. D., Wadsworth, H. L., and Rapoport, B. (1990) Transcrip-tional regulation of ferritin H messenger RNA levels in FRTL5 rat thyroid cells by thyrotropin. J. Biol Chem 265, 666–670.
Chinsky, J. M., Maa, M. C., Ramamurthy, V., and Kellems, R. E. (1989) Adenosine deaminase gene expression Tissue-dependent regulation of tran-scriptional elongation. J. Biol. Chem 264, 14,561–14,565
Saini, K., Thomas, P., and Bhandari, B. (1990) Hormonal regulation of stabil-ity of glutamine synthetase mRNA in cultured 3T3-L1 adipocytes. Biochem. J. 267, 241–244.
Brock, M. L. and Shapiro, D. J. (1983) Estrogen stabilizes vitellogenin mRNA against cytoplasmic degradation. Cell 34, 207–214
Antrast, J., Lasnier, F., and Pairault, J. (1991) Adipsin gene expression in 3T3-F442A adipocytes is post-transcriptionally down-regulated by retinoic acid J. Biol.Chem. 266, 1157–1161.
Jefferson, D. M., Clayton, D. F., Darnell, J. E. Jr, and Reid, L. M. (1986) Posttranscriptional modulation of gene expression in cultured rat hepatocytes Mol. Cell Biol. 4, 1929–1934
Hod, Y. and Hanson, R. W.(1988) CyclicAMPstabihzesthemRNAforphosphoenol pyruvate carboxykinase (GTP) against degradation, J. Biol.Chem. 263, 7747–7752.
Gordon, D. A., Shelness, G. J., Nicosia, M., and Williams, D. L. (1988) Estro-gen-induced destabilization of yolk precursor protein mRNA in avian liver J. Biol. Chem 263, 2625–2631.
Srivastava, R. A. K., Jiao, S., Tang, J., Pfleger, B., Kitchens, R. T., and Schonfeld, G. (1991) In vivo regulation of low density lipoprotein receptor and apolipoprotein B gene expresion by dietary fatty acids and dietary choles-terol in inbred strains of mice, Biochim. Biophys. Acta 1086, 29–43.
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Srivastava, R.A.K., Schonfeld, G. (1994). Measurements of Rate of Transcription in Isolated Nuclei by Nuclear “Run-Off” Assay. In: Harwood, A.J. (eds) Protocols for Gene Analysis. Methods in Molecular Biology, vol 31. Humana Press. https://doi.org/10.1385/0-89603-258-2:281
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DOI: https://doi.org/10.1385/0-89603-258-2:281
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