Abstract
The potency of gel electrophoresis to analyze conformational transitions of nucleic acids has opened up a new field of applications. Helix coil transitions and, at a refined level, minor variations because of mutations and mismatches, may be studied experimentally by solvent -gradient and temperature-gradient gel electrophoresis. The latter method is the subject of this chapter.
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© 1994 Humana Press Inc., Totowa, NJ
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Henco, K., Harders, J., Wiese, U., Riesner, D. (1994). Temperature Gradient Gel Electrophoresis (TGGE) for the Detection of Polymorphic DNA and RNA. In: Harwood, A.J. (eds) Protocols for Gene Analysis. Methods in Molecular Biology, vol 31. Humana Press. https://doi.org/10.1385/0-89603-258-2:211
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DOI: https://doi.org/10.1385/0-89603-258-2:211
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