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The Simultaneous Isolation of RNA and DNA from Tissues and Cultured Cells

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Protocols for Gene Analysis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 31))

Abstract

Many techniques are currently available that allow the isolation of DNA (1–7) or RNA (8–23), but such methods allow only the purification of one type of nucleic acid at the expense of the other. Frequently, when cellular material is limiting, it is desirable to isolate both RNA and DNA from the same source. Such is the case for biopsy specimens, primary cell lines, or manipulated embryonic stem cells.

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Author information

Authors and Affiliations

  1. Department of Genetics, Research Institute, The Hospital for Sick Children, Toronto, Ontario, Canada

    Frank Merante

  2. Department of Biochemistry, Erindale College, University of Toronto, Mississauga, Ontario, Canada

    Sandeep Raha

  3. Departments of Biochemistry and Chemistry, Erindale College, University of Toronto, Mississauga, Ontario, Canada

    Juta K. Reed

  4. Department of Zoology, Erindale College, University of Toronto, Mississauga, Ontario, Canada

    Gerald Proteau

Authors
  1. Frank Merante
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  2. Sandeep Raha
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  3. Juta K. Reed
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  4. Gerald Proteau
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Editor information

Editors and Affiliations

  1. Medical Research Council, Cambridge, UK

    Adrian J. Harwood

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© 1994 Humana Press Inc, Totowa, NJ

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Merante, F., Raha, S., Reed, J.K., Proteau, G. (1994). The Simultaneous Isolation of RNA and DNA from Tissues and Cultured Cells. In: Harwood, A.J. (eds) Protocols for Gene Analysis. Methods in Molecular Biology, vol 31. Humana Press. https://doi.org/10.1385/0-89603-258-2:113

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  • DOI: https://doi.org/10.1385/0-89603-258-2:113

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-258-3

  • Online ISBN: 978-1-59259-518-1

  • eBook Packages: Springer Protocols

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