Construction of Linker-Scanning Mutations by Oligonucleotide Ligation

  • Grace M. Hobson
  • Patricia P. Harlow
  • Pamela A. Benfield
Part of the Methods in Molecular Biology book series (MIMB, volume 31)


The purpose of linker-scanning mutagenesis is to create a series of mutant molecules in which individual sections are sequentially replaced with the same “neutral” mutant sequence (see Fig. 1). Typically the technique has been applied to DNA and has most commonly been used to determine the cw-regulatory roles of upstream flanking regions of genes. The technique has several advantages over more random mutational analysis:


Alanine Scanning Mutagenesis Synthetic Fragment Mutant Molecule Upstream Flank Region Endonuclease Restriction Site 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Humana Press Inc, Totowa, NJ 1994

Authors and Affiliations

  • Grace M. Hobson
    • 1
  • Patricia P. Harlow
    • 2
  • Pamela A. Benfield
    • 2
  1. 1.Alfred L DuPont InstituteWilmington
  2. 2.The DuPont Merck Pharmaceutical Company, Experimental StationWilmington

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