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DNA-Protein Interactions pp 357–370Cite as

Reconstitution of Protein-DNA Complexes for Crystallization

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 30))

Abstract

An increasing number of structural studies are aimed at identifying the principles that govern protein-DNA recognition in gene regulation (1). This work depends on the successful reconstitution of protein-DNA complexes from their purified components. X-ray crystallography and two-dimensional NMR techniques require large amounts of pure protein and DNA. These can be supplied through expression in bacteria of the cDNA coding for intact proteins or their smaller DNA-binding domains and the automated chemical synthesis of DNA in the laboratory.

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Author information

Authors and Affiliations

  1. Howard Hughes Medical Institute, Harvard University, Cambridge, MA

    Rachel M. Conlin

  2. Howard Hughes Medical Institute, Harvard University, Boston, MA

    Raymond S. Brown

Authors
  1. Rachel M. Conlin
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  2. Raymond S. Brown
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Editor information

Editors and Affiliations

  1. School of Biological Sciences; University of Portsmouth, Portsmouth, UK

    G. Geoff Kneale

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© 1994 Humana Press Inc.

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Conlin, R.M., Brown, R.S. (1994). Reconstitution of Protein-DNA Complexes for Crystallization. In: Geoff Kneale, G. (eds) DNA-Protein Interactions. Methods in Molecular Biology™, vol 30. Humana Press. https://doi.org/10.1385/0-89603-256-6:357

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  • DOI: https://doi.org/10.1385/0-89603-256-6:357

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-256-9

  • Online ISBN: 978-1-59259-517-4

  • eBook Packages: Springer Protocols

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