Abstract
A very powerful method of probing DNA-protein interactions is the technique of site-directed mutagenesis. Using this approach one can introduce specific amino acid changes at any given position in the amino acid sequence of a DNA-binding protein, and test the functional consequences of these mutations in vitro. Furthermore, it is also possible to investigate the resulting mutations in vivo if a suitable phenotypic change can be measured. If so, strategies that introduce random base changes to a given codon (or indeed to a larger region of the protein) are particularly valuable.
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© 1994 Humana Press Inc.
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O’Donohue, M.J., GeoffKneale, G. (1994). Site-Directed and Site-Saturation Mutagenesis Using Oligonucleotide Primers. In: Geoff Kneale, G. (eds) DNA-Protein Interactions. Methods in Molecular Biology™, vol 30. Humana Press. https://doi.org/10.1385/0-89603-256-6:211
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DOI: https://doi.org/10.1385/0-89603-256-6:211
Publisher Name: Humana Press
Print ISBN: 978-0-89603-256-9
Online ISBN: 978-1-59259-517-4
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