Abstract
Chemical modification is a powerful tool for investigating the accessibility and function of specific amino acids within folded proteins. It has provided significant information regarding the role of different amino acids at the binding sites of numerous enzymes and DNA binding proteins. This information has frequently been used to plan subsequent site-directed mutagenesis experiments. Additionally, the data from chemical modification experiments complements that from crystallographic and NMR data in elucidating the residues located at the DNA binding site.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Lundblad, R. and Noyes, M. (1984) in Chemical Reagents for Protein Modifcation I and II, CRC, Boca Raton, FL.
Riordan, J, Sokolovsky, M., and Vallee, B. (1967) Environmentally sensitive tyrosine residues. Nitration with tetramtromethane. Biochemistry 6,358.
Anderson, R., Nakashima, Y., and Coleman, J. (1975) Chemical modification of functional residues of the Fd gene 5 DNA-binding protein. Biochemistry 14,907–917.
Sokolovsky, M., Riordan, J, and Vallee, B. (1966) Tetranitromethane. A reagent for the nitration of tyrosyl residues in proteins. Biochemistry 5,3582–3589.
Sokolovsky, M., Harell, G., and Rrordan, J. (1969) Reaction of tetranitromethane with sulphydryl groups in proteins. Biochemistry 8,4740–4745.
Dimicoli, J. and Helene, C. (1974) Interaction of aromatic residues of proteins with nucleic acids I and II. Biochemistry 13,714–730.
Sokolovsky, M., Riordan, J., and Vallee, B. (1967) Conversion of 3-nitrotyrosine to 3-ammotyrosine in peptides and proteins. Biochem. Biophys.Res. Commun. 27,20.
Plyte, S. E. and Kneale, G. G. (1991) Mapping the DNA binding site of the Pfl gene 5 protein. Protein Eng. 45), 553–560.
Anderson, R. and Coleman, J. (1975) Physiochemical properties of DNA-binding proteins: Gene 32 protein of T4 and Escherichia coli unwinding protein.Biochemistry 14,5485–5491.
Plyte, S. (1990) Ph.D. Thesis. The biochemical and biophysical characterization of the PFl gene 5 protein and its complex with nucleic acids. Portsmouth Polytechnic, Portsmouth, UK.
Martinson, H. and McCarthy, B (1975) Histone-histone associations within chromatin. Cross-linking studies using tetramtromethane. Biochemistry 14,1073–1078.
Williams, J. and Lowe, J. (1971) The crosslinking of tyrosine with tetranitromethane.Biochem. J. 121,203–209.
Bruice, T., Gregory, M., and Walters, S. (1968) Reactions of tetranitromethane (I) Kinetics and mechanism of nitration of phenols by tetranitromethane. J.Am. Chem. Sot. 90, 1612–1619.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1994 Humana Press Inc.
About this protocol
Cite this protocol
Plyte, S.E. (1994). Nitration of Tyrosine Residues in Protein-Nucleic Acid Complexes. In: Geoff Kneale, G. (eds) DNA-Protein Interactions. Methods in Molecular Biology™, vol 30. Humana Press. https://doi.org/10.1385/0-89603-256-6:151
Download citation
DOI: https://doi.org/10.1385/0-89603-256-6:151
Publisher Name: Humana Press
Print ISBN: 978-0-89603-256-9
Online ISBN: 978-1-59259-517-4
eBook Packages: Springer Protocols