Abstract
RNA dot hybridizations were first described by Kafatos et al. (1). They allow rapid detection of transcription from a number of mRNA populations and are particularly useful in the initial characterization of clones derived from differentially expressed genes. Where accurate quantification of transcription is necessary, or many samples have to be handled, filtration manifold systems are available, such as the Millipore (Bedford, MA) MilliBlot system, that use a vacuum source to transfer nucleic acid to filter.
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References
Kafatos, F. C., Jones, C. W., and Efstratiadis, A. (1979) Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedure. Nucleic Acids Res. 7, 1541–1552.
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© 1994 Humana Press Inc., Totowa, NJ
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Hodge, R. (1994). Preparation of RNA Dot Blots. In: Isaac, P.G. (eds) Protocols for Nucleic Acid Analysis by Nonradioactive Probes. Methods in Molecular Biology™, vol 28. Humana Press. https://doi.org/10.1385/0-89603-254-X:55
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DOI: https://doi.org/10.1385/0-89603-254-X:55
Publisher Name: Humana Press
Print ISBN: 978-0-89603-254-5
Online ISBN: 978-1-59259-515-0
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