Abstract
DNA probes can be labeled using the modified nucleotide, fluo-rescein-11-dUTP, and subsequently detected with enhanced chemiluminescence (1) using an antifluorescein antibody conjugated to horseradish peroxidase (2). The use of enhanced chemiluminescence avoids the fading of results associated with some colormetric detection procedures, used with nonradioactive labeling and detection systems. Hard copy results showing excellent resolution are produced.
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References
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© 1994 Humana Press Inc., Totowa, NJ
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Harvey, B.M., Wheeler, C.B. (1994). Hybridization and Detection of Fluorescein-Labeled DNA Probes Using Enhanced Chemiluminescence. In: Isaac, P.G. (eds) Protocols for Nucleic Acid Analysis by Nonradioactive Probes. Methods in Molecular Biology™, vol 28. Humana Press. https://doi.org/10.1385/0-89603-254-X:135
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DOI: https://doi.org/10.1385/0-89603-254-X:135
Publisher Name: Humana Press
Print ISBN: 978-0-89603-254-5
Online ISBN: 978-1-59259-515-0
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