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Detection of Digoxigenin-Labeled DNA Probes Hybridized to Plant Chromosomes In Situ

  • Ilia J. Leitch
  • J. S. Pat Heslop-Harrison
Part of the Methods in Molecular Biology™ book series (MIMB, volume 28)

Abstract

Digoxigenin is widely used as a nonradioactive label for in situ hybridization to locate DNA sequences along chromosomes in plants (e.g., 1, 2, 3) and animals. In many cases, its use is similar to biotin ( chapter 25, or e.g., 4), but digoxigenin labels may give lower unspecific background signal and therefore enable more efficient detection of short or low copy sequences. Digoxigenin is usually incorporated into the DNA enzymatically by nick translation, random priming, polymerase chain reaction (PCR), or end labeling using the modified nucleotide digoxigenin-11-dUTP (see  chapter 10 and  chapter 11).

Keywords

Hybridization Signal Hydrogen Peroxide Solution Detection Buffer Tungstosilicic Acid Posthybridization Wash 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

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Copyright information

© Humana Press Inc., Totowa, NJ 1994

Authors and Affiliations

  • Ilia J. Leitch
    • 1
  • J. S. Pat Heslop-Harrison
    • 1
  1. 1.Karyobiology Group, Department of Cell BiologyJohn Innes CentreNorwichUK

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