Abstract
Part A. Protein Kinase-Mediated Phosphorylation Events
Protein kinases (ATP:protein phosphotransferases) regulate a wide range of cellular events, including the transduction of signals leading to cell growth. Although the protein kinase superfamily encompasses a large and structurally diverse group of enzymes, protein and DNA sequencing data indicate that they share a common evolutionary origin with a particularly highly conserved catalytic core structure and function. At present, the well characterized protein kinases (PK) can be divided into four main subclasses
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Garrison, J. C (1983) Measurement of hormone stimulated protein phosphorylation in intact cells. Methods Enzymol. 99, 20–36.
Yin Jen Wang, J. and Baltimore, D. (1983) Characterization of the abelson murine leukemia virus-encoded tyrosine-specific protein kinase. Methods Enzymol. 99, 373–378.
Roach, P. J (1984) Protein kinases Methods Enzymol 107, 81–101
Cooper, J. A, Sefton, B. M., and Hunter, T. (1983) Detection and quantitation of phosphotyrosine in proteins. Methods Enzymol. 99, 387–402.
Cohen, S. (1983) Purification of the receptor for epidermal growth factor from A-431 cells: its function as a tyrosyl kinase. Methods Enzymol. 99, 379–3
Swamp, G., Cohen, S., and Garbers, D L. (1981) Selective dephosphorylation of proteins containing phosphotyrosine by alkaline phosphatase J Biol Chem. 256, 8197–8201.
Ushiro, H and Cohen, S. (1980) Identification of phosphotyrosine as a product of epidermal growth factor-activated protein kinase in A-431 cell mem branes. J. Biol. Chem. 255, 8363–8365.
Kikkawa, U, Kishimoto, A., and Nishizuka, Y. (1989) The protein kinase C family: heterogeneity and its implication. Ann. Rev. Bwchem. 58, 31–44.
Girard, P. R., Mazzei, G. J., Wood, J G., and Kuo, J. F. (1985) Polyclonal antibodies to phosphohpid/Ca2+-dependent protein kinase and lmmunocy-tochemical localization of the enzyme in rat brain. Proc. Natl. Acad. Sci. USA 82, 3030–3034.
Ballester, R and Rosen, O. M. (1985) Fate of immunoprecipitable PKC in GH3 cells treated with phorbol 12-myristate 13-acetate. J. Biol. Chem 260, 15,194–15,199.
Shearman, M. S., Berry, N., Oda, F, Ase, K., Kikkawa, U., and Nishizuka, Y. (1988) Isolation of protein kinase C subspecies from a preparation of human T lymphocytes. FEBS Lett. 234, 387–391.
Blackshear, P. J, Witters, L. A., Girard, P. R, Kuo, J. F., and Quamo, S. N (1985) Growth factor-stimulated protein phosphorylation in 3T3-L1 cells J. Biol. Chem. 260, 13,304–13,315.
Girard, P. R., Stevens, V. L., Blackshear, P. J., Merrill, A. H., Wood, J. G., and Kuo, J. F. (1987) Immunocytochemical evidence for phorbol ester-induced directional translocation of protein kinase C in HL60, K562, CHO, and E75KS cells: possible role in differentiation Cancer Res. 47, 2892–2898.
Coussens, L. Parker, P. J, Rhee, L, Yang-Feng, T. L, Chen, E, Waterfield, M., Francke, U., and Ullrich, A (1986) Multiple distinct forms of bovine and human protein kinase C suggests diversity in cellular signalling pathways Science 223, 859–866.
Brandt, S. J., Niedel, J. E, Bell, R M., and Young, W S. (1987) Distinct patterns of expression of different protein kinase C mRNAs in rat tissue Cell 49, 67–73.
Solanki, V. and Slaga, T. T. (1981) Specific binding of phorbol ester tumor promoters to intact primary epidermal cells from Senear mice. Proc. Natl. Acad. Sci. USA 78, 2549–2553.
Bell, R M, Hannun, Y., and Loomis, C. (1986) Mixed micelle assay of protein kinase C. Methods Enzymol 124, 353–359
Pelech, S. L., Meier, K E., and Krebs, E G. (1986) Rapid microassay for PKC translocation in Swiss 3T3 cells. Biochemistry 25, 8348–8353
Author information
Authors and Affiliations
Rights and permissions
Copyright information
© 1994 Humana Press Inc. Totowa, NJ
About this protocol
Cite this protocol
Harnett, M.M. (1994). Assay of Protein Kinases and Protein Phosphorylation. In: Biomembrane Protocols. Methods in Molecular Biology, vol 27. Springer, Totowa, NJ. https://doi.org/10.1385/0-89603-250-7:213
Download citation
DOI: https://doi.org/10.1385/0-89603-250-7:213
Publisher Name: Springer, Totowa, NJ
Print ISBN: 978-0-89603-250-7
Online ISBN: 978-1-59259-514-3
eBook Packages: Springer Protocols