Abstract
Cyclic adenosine 3′,5′-monophosphate (cAMP) and cyclic guanosine 3′,5′-monophosphate (cGMP) have between them been implicated in the mediation of many physiological phenomena. Over the last 30 years, a number of techniques have been employed to assess changes in the amounts of these two molecules. Many, however, suffered from lack of sensitivity and/or ease of performance. Since the early 1970s, two methods have been developed that to a large extent overcome these problems. The first is based on competitive binding to endogenous proteins (1). The second method is a competitive radioimmunoassay that, with minor modifications, can be used to detect levels of cyclic nucleotides down to 1 fmol/assay tube; this is discussed in detail below (2,3). The method is equally applicable to the measurement of all cyclic nucleotides; however, for clarity only that for cAMP is described.
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References
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© 1994 Humana Press Inc. Totowa, NJ
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Whitley, G.S.J. (1994). Extraction and Assay of Cyclic Nucleotides. In: Biomembrane Protocols. Methods in Molecular Biology, vol 27. Springer, Totowa, NJ. https://doi.org/10.1385/0-89603-250-7:189
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DOI: https://doi.org/10.1385/0-89603-250-7:189
Publisher Name: Springer, Totowa, NJ
Print ISBN: 978-0-89603-250-7
Online ISBN: 978-1-59259-514-3
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