Abstract
The synthesis, characterization, and use of fluorescently labeled lipid probes to follow transport in living cells at a microscopic level have greatly expanded our knowledge of intracellular lipid trafficking. Although lipids containing a number of covalently attached fluorophores have been synthesized, most transport studies have used lipids labeled with 4-nitrobenzo-2-oxa-l,3-diazole (NBD).** Several lines of evidence suggest that NBD-labeled lipids faithfully mimic their native counterparts (see ref. 1 for review). For example:
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© 1994 Humana Press Inc. Totowa, NJ
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Sleight, R.G. (1994). Fluorescent Glycerolipid Probes. In: Biomembrane Protocols. Methods in Molecular Biology, vol 27. Springer, Totowa, NJ. https://doi.org/10.1385/0-89603-250-7:143
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DOI: https://doi.org/10.1385/0-89603-250-7:143
Publisher Name: Springer, Totowa, NJ
Print ISBN: 978-0-89603-250-7
Online ISBN: 978-1-59259-514-3
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