Abstract
The neurotrophins are expressed in many cell types including fibroblasts, Schwann cells, salivary gland secretory cells, glia, and neurons. Neurotrophin gene expression in these cells is regulated developmentally, and once switched on it is regulated by diverse extracellular signals and stimuli. Most cell types secrete neurotrophins constitutively.
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References
Allen J. A. and Hemrich G. (1987) unpublished.
Allen N. D., Norris M. L., and Surani M. (1990) Epigenetic control of transgene expression and imprinting by genotype-specific modifiers. Cell 61, 853–861.
Amaya E., Musci T. J., and Kirschner M. W. (1991) Expression of a dominant negative mutant of the FGF receptor disrupts mesodenn formation in Xenopus embryos. Cell 66, 257–270.
Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Smith J. A., Seidman J. G., and Struhl J. G., and Green K., eds. (1987) Current Protocols in Molecular Biology, Publishing Associates and Wiley-Interscience, New York.
Black M., Pope L., Lefebvre F.-A., and Walker P (1989) Truodothyronine mcreases nerve growth factor gene transcription in female mouse submandibular gland Endocrine Society Annual Meeting Abstract 269, Seattle.
Borsani G., Pizzuti A., Rugarh E. I, Falini A., Silani V., Sidoli A., Scarlato G., and Baralle F. E. (1990) cDNA sequence of human β-NGF. Nucl. Acids Res. 18, 4020.
Breakefield X. O., Orloff G., Castiglione C., Coussens L., Axelrod F. B., and Ullrich A. (1984) Structural gene for β-nerve growth factor not defective in familial dysautonomia. Proc. Natl. Acad. Sci. USA 81, 4213–4216.
Burke J. M., Esherick J. S., Burfeind W. R., and King J. L. (1990) A 3’ splice site-binding sequence in the catalytic core of a group I intron. Nature 344, 80–82.
Crete N., Delabar J. M., Sinet P. M., and Creau-Goldberg N. (1991) Accurate evaluation of the sizes of DNA fragments (from 30 to 4700 kb) in pulse field gel electrophoresis. BioTechnques 11, 711–718.
Curran T. and Franza B. R. (1988) Fos and Jun: the Al’-1 connection. Cell 55, 395–397.
Davis L G., Dibner M. D., and Battey J. F. (1986) Basic Methods in Molecular Biology, Elsevier, New York.
D’Mello S. R. and Heinrich G. (1991a) Nerve growth factor gene expression: mvolvement of a downstream AP-1 element in basal and modulated transcription. Mol. Cell. Neurosci. 2, 157–167.
D’Mello S. R. and Heinrich. G. (1991b) Structural and functional identification of regulatory regions surrounding the nerve growth factor gene promoter. Mol. Brain Res. 11, 255–264.
Donaghue M. J., Merlie J. P., and Sanes J. R. (1991) A rostrocaudal gradient of transgenic expression in adult mouse skeletal muscle. Soc. Neurosci Abstr. 17, Part 1, p. 10.
Dracopoli N. C., Rose E., Whitfield G. K, Guidon Jr. P. T., Bale S. J., Chance P. A., Kourides I. A., and Housman D. E. (1988) Two thyroid hormone regulated genes the β-subunit of nerve growth factor (NGFβ) and thyroid hormone stimulating hormone (TSHJβ) are located less than 310 kb apart in both human and mouse genomes. Genomics 3, 161–167.
Edwards R. H., Rutter J. W., and Hanahan D. (1989) Directed expression of NGF to pancreatic β cells in transgenic mice leads to selective hyperinnervation of the islets. Cell 58, 161–170.
Edwards R. H., Selby M. J., and Rutter W. J. (1986) Differential RNA splicing predicts two distmct nerve growth factor precursors. Nature 319, 784–787.
Federoff H. J., Geschwind M. D., Geller A. I., and Kessler J. A. (1991) Expression of NGF in vivo from a defective HSV-1 vector, alters the response to peripheral nerve axotomy. Soc. Neurosci. Abstr. 17, Part 1, p. 711.
Francke U., De Martmville B., Coussens L., and Ullrich A. (1983) The human gene for the β-subunit of nerve growth factor is located on the proximal short arm of chromosome 1. Science 222, 1248–1251.
Frohman M. A., Dush M. K., and Martin G. R. (1988) Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. Proc. Natl. Acad. Sci. USA 85, 8998–9002.
Gardiner K., Laas W., and Patterson D. (1988) Fractionation of large mammalian DNA restriction fragments using vertical pulsed field gradient gel electrophoresis. Som. Cell Mol. Genet. 12, 185–195.
Hallbook F., Ibanez C. F., and Persson H. (1991) Evolutionary studies of the nerve growth factor family reveal a novel member abundantly expressed in Xenopus ovary. Neuron 6, 845–858.
Hengerer B., Lindholm, D., Heumann R., Ruther U., Wagner E. W., and Thoenen H. (1990) Lesion-induced increase in nerve growth factor mRNA is mediated by c-fos. Proc. Natl. Acad. Sci. USA 87, 3899–3903.
Innis M. A., Gelfand D. H., Sninsky J. J., and White T, J., eds. (1990) PCR Protocols: a guide methods and applications, Academic, Boston, MA.
Jackson P. D. and Felsenfeld G. (1987) In vivo footprmting of specific protern-DNA interactions. Methods in Enzymology 152, 735–755.
Jackson R. J. and Standart N. (1990) Do the poly(A) tail and 3’ untranslated region control mRNA translation? Cell 62, 15–24.
Kationaga J. T. and Tjian R(1986) Affinity purification of sequence-specific DNA binding proteins. Proc. Natl. Acad. Sci USA 83, 5889–5893.
Lindholm D., Heumann R., Hengerer B., and Thoenen H. (1988) Interleukin-1 increases stability and transcription of mRNA encoding nerve growth factor in cultured rat fibroblasts. J. Biol. Chem. 263, 16,348–16,351.
Maisonpierre P. C., Le Beau M. M., Espinosa III R., Ip N. Y., Belluscio L., De La Monte S. M., Squinto S., Furth M. E., and Yancopoulos G. D. (1991) Human rat brain-derived neurotrophic factor neurotorphin-3: gene structure, distributions, and chromosomal localizations. Genomics 10, 558–568.
Maxam A. and Gilbert W. (1980) Sequencing end-labeled DNA with base-specific chemical cleavages. Methods in Enzymology 65, 499–560.
Moazed D., Stern S., and Noller H. F. (1986) Rapid chemical probing of conformation in 16S ribosomal RNA and 30S ribosomal subunits using primer extension. J. Mol. Biol. 187, 399–416.
Mueller P. R. and Wolf B. (1989) In vivo footprinting of a muscle-specific enhancer by ligation mediated PCR. Science 246, 780–786. (This laboratory also makes an updated printed protocol for in vivo footprinting available on request.)
O/rsbrien T. and Lis J. T. (1991) RNA polymerase II pauses at the 5’ end of the transcriptionally induced Drosophila-hsp70 gene. Mol. Cell. Biol. 11, 5285–5290.
Onteniente B., Horellou P., Makeh I., Lisovski E., Grimber G., Briand P., Mallet J., and Peschanski M A (1991) A c-fos-NGF transgenic mice. New perspectives in the study of the factor. Sot. Neurosci. Abstr. 17, Part 1, p. 1117.
Ozcelik T., Rosenthal A., and Francke U. (1991) Chromosomal mapping of brain-derived neurotrophic factor and neurotrophin-3 genes in man and mouse. Genomics 10, 569–575.
Ross J. and Kobs G. (1986) H4 histone messenger RNA decay in cell-free extracts initiates at or near the 3’ terminus and proceeds 3’ to 5’. J. Mol. Biol. 188, 579–593.
Rossant J. (1990) Manipulating the mouse genome: implications for neuro-biology. Neuron 4, 323–334.
Russo A. F., Crenshaw III E. B., Lira S. A., Simmons D. M., Swanson L. W., and Rosenfeld M. G. (1988) Neuronal expression of chuneric genes in transgenic mice. Neuron 1, 311–320.
Saltzman A. G. and Wemmann R. (1989) Promoter specrfrcity and modulation of RNA polymerase II transcription. FASEB J. 3, 1723–1733.
Sambrook J., Fritsch E. F., and Maniatis T. (1989) Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
Sariola H., Saarma M., Sainio K., Arumae U., Palgi J., Vaahtokari A., Thesleff I., and Karavanov A. (1991) Dependence of kidney morphogenesis on the expression of nerve growth factor receptor. Science 254, 571–573.
Selden R. F., Howie K. B., Rowe M. E., Goodman H. M., and Moore D. D. (1986) Human growth hormone as a reporter gene in regulation stud-ies employing transient gene expression. Mol. Cell. Biol. 6, 3173–3179.
Shaw G. and Kamen K. (1986) A conserved AU sequence from the 3’ untranslated region of GM-CSF mRNA mediates selective mRNA degradation. Cell 46, 659–667.
Sternberg N. (1990) Bacteriophage Pl cloning system for the isolation, amplification, and recovery of DNA fragments as large as 100 kilobase pairs. Proc. Natl. Acud. Sei. USA 87, 103–107.
Ullrich A., Gray A., Berman C., and Dull T J. (1983) Human beta-nerve growth factor gene sequence highly homologous to that of mouse. Nature 303, 821–825.
Wickens M. and Stephenson P. (1984) Role of the conserved AAUAAA sequence: four AAUAAA point mutations prevent messenger RNA 3’ end formation. Sctence 226, 1045–1051.
Yashpe J. and Halvorson H. 0. (1976) /gb-D-galactosidase activity m single yeast ceils during cell cycle in Saccharomyces lactis. Science 191, 1283.
Zheng M. and Heinrich G. (1988) Structural and functional analysis of the promoter region of the nerve growth factor gene. Mol. Brain Res. 3, 133–140.
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Heinrich, G., Martin, S. (1993). Molecular Analysis of Neurotrophin Genes. In: Boulton, A.A., Baker, G.B., Hefti, F. (eds) Neurotrophic Factors. Neuromethods, vol 25. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-249-3:107
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DOI: https://doi.org/10.1385/0-89603-249-3:107
Publisher Name: Humana Press, Totowa, NJ
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