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Molecular Analysis of Neurotrophin Genes

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Neurotrophic Factors

Part of the book series: Neuromethods ((NM,volume 25))

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Abstract

The neurotrophins are expressed in many cell types including fibroblasts, Schwann cells, salivary gland secretory cells, glia, and neurons. Neurotrophin gene expression in these cells is regulated developmentally, and once switched on it is regulated by diverse extracellular signals and stimuli. Most cell types secrete neurotrophins constitutively.

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References

  • Allen J. A. and Hemrich G. (1987) unpublished.

    Google Scholar 

  • Allen N. D., Norris M. L., and Surani M. (1990) Epigenetic control of transgene expression and imprinting by genotype-specific modifiers. Cell 61, 853–861.

    Article  PubMed  CAS  Google Scholar 

  • Amaya E., Musci T. J., and Kirschner M. W. (1991) Expression of a dominant negative mutant of the FGF receptor disrupts mesodenn formation in Xenopus embryos. Cell 66, 257–270.

    Article  PubMed  CAS  Google Scholar 

  • Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Smith J. A., Seidman J. G., and Struhl J. G., and Green K., eds. (1987) Current Protocols in Molecular Biology, Publishing Associates and Wiley-Interscience, New York.

    Google Scholar 

  • Black M., Pope L., Lefebvre F.-A., and Walker P (1989) Truodothyronine mcreases nerve growth factor gene transcription in female mouse submandibular gland Endocrine Society Annual Meeting Abstract 269, Seattle.

    Google Scholar 

  • Borsani G., Pizzuti A., Rugarh E. I, Falini A., Silani V., Sidoli A., Scarlato G., and Baralle F. E. (1990) cDNA sequence of human β-NGF. Nucl. Acids Res. 18, 4020.

    Google Scholar 

  • Breakefield X. O., Orloff G., Castiglione C., Coussens L., Axelrod F. B., and Ullrich A. (1984) Structural gene for β-nerve growth factor not defective in familial dysautonomia. Proc. Natl. Acad. Sci. USA 81, 4213–4216.

    Article  PubMed  CAS  Google Scholar 

  • Burke J. M., Esherick J. S., Burfeind W. R., and King J. L. (1990) A 3’ splice site-binding sequence in the catalytic core of a group I intron. Nature 344, 80–82.

    Article  PubMed  CAS  Google Scholar 

  • Crete N., Delabar J. M., Sinet P. M., and Creau-Goldberg N. (1991) Accurate evaluation of the sizes of DNA fragments (from 30 to 4700 kb) in pulse field gel electrophoresis. BioTechnques 11, 711–718.

    CAS  Google Scholar 

  • Curran T. and Franza B. R. (1988) Fos and Jun: the Al’-1 connection. Cell 55, 395–397.

    Article  PubMed  CAS  Google Scholar 

  • Davis L G., Dibner M. D., and Battey J. F. (1986) Basic Methods in Molecular Biology, Elsevier, New York.

    Google Scholar 

  • D’Mello S. R. and Heinrich G. (1991a) Nerve growth factor gene expression: mvolvement of a downstream AP-1 element in basal and modulated transcription. Mol. Cell. Neurosci. 2, 157–167.

    Article  Google Scholar 

  • D’Mello S. R. and Heinrich. G. (1991b) Structural and functional identification of regulatory regions surrounding the nerve growth factor gene promoter. Mol. Brain Res. 11, 255–264.

    Article  PubMed  Google Scholar 

  • Donaghue M. J., Merlie J. P., and Sanes J. R. (1991) A rostrocaudal gradient of transgenic expression in adult mouse skeletal muscle. Soc. Neurosci Abstr. 17, Part 1, p. 10.

    Google Scholar 

  • Dracopoli N. C., Rose E., Whitfield G. K, Guidon Jr. P. T., Bale S. J., Chance P. A., Kourides I. A., and Housman D. E. (1988) Two thyroid hormone regulated genes the β-subunit of nerve growth factor (NGFβ) and thyroid hormone stimulating hormone (TSHJβ) are located less than 310 kb apart in both human and mouse genomes. Genomics 3, 161–167.

    Article  PubMed  CAS  Google Scholar 

  • Edwards R. H., Rutter J. W., and Hanahan D. (1989) Directed expression of NGF to pancreatic β cells in transgenic mice leads to selective hyperinnervation of the islets. Cell 58, 161–170.

    Article  PubMed  CAS  Google Scholar 

  • Edwards R. H., Selby M. J., and Rutter W. J. (1986) Differential RNA splicing predicts two distmct nerve growth factor precursors. Nature 319, 784–787.

    Article  PubMed  CAS  Google Scholar 

  • Federoff H. J., Geschwind M. D., Geller A. I., and Kessler J. A. (1991) Expression of NGF in vivo from a defective HSV-1 vector, alters the response to peripheral nerve axotomy. Soc. Neurosci. Abstr. 17, Part 1, p. 711.

    Google Scholar 

  • Francke U., De Martmville B., Coussens L., and Ullrich A. (1983) The human gene for the β-subunit of nerve growth factor is located on the proximal short arm of chromosome 1. Science 222, 1248–1251.

    Article  PubMed  CAS  Google Scholar 

  • Frohman M. A., Dush M. K., and Martin G. R. (1988) Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. Proc. Natl. Acad. Sci. USA 85, 8998–9002.

    Article  PubMed  CAS  Google Scholar 

  • Gardiner K., Laas W., and Patterson D. (1988) Fractionation of large mammalian DNA restriction fragments using vertical pulsed field gradient gel electrophoresis. Som. Cell Mol. Genet. 12, 185–195.

    Article  Google Scholar 

  • Hallbook F., Ibanez C. F., and Persson H. (1991) Evolutionary studies of the nerve growth factor family reveal a novel member abundantly expressed in Xenopus ovary. Neuron 6, 845–858.

    Article  PubMed  CAS  Google Scholar 

  • Hengerer B., Lindholm, D., Heumann R., Ruther U., Wagner E. W., and Thoenen H. (1990) Lesion-induced increase in nerve growth factor mRNA is mediated by c-fos. Proc. Natl. Acad. Sci. USA 87, 3899–3903.

    Article  PubMed  CAS  Google Scholar 

  • Innis M. A., Gelfand D. H., Sninsky J. J., and White T, J., eds. (1990) PCR Protocols: a guide methods and applications, Academic, Boston, MA.

    Google Scholar 

  • Jackson P. D. and Felsenfeld G. (1987) In vivo footprmting of specific protern-DNA interactions. Methods in Enzymology 152, 735–755.

    Article  PubMed  CAS  Google Scholar 

  • Jackson R. J. and Standart N. (1990) Do the poly(A) tail and 3’ untranslated region control mRNA translation? Cell 62, 15–24.

    Article  PubMed  CAS  Google Scholar 

  • Kationaga J. T. and Tjian R(1986) Affinity purification of sequence-specific DNA binding proteins. Proc. Natl. Acad. Sci USA 83, 5889–5893.

    Article  Google Scholar 

  • Lindholm D., Heumann R., Hengerer B., and Thoenen H. (1988) Interleukin-1 increases stability and transcription of mRNA encoding nerve growth factor in cultured rat fibroblasts. J. Biol. Chem. 263, 16,348–16,351.

    PubMed  CAS  Google Scholar 

  • Maisonpierre P. C., Le Beau M. M., Espinosa III R., Ip N. Y., Belluscio L., De La Monte S. M., Squinto S., Furth M. E., and Yancopoulos G. D. (1991) Human rat brain-derived neurotrophic factor neurotorphin-3: gene structure, distributions, and chromosomal localizations. Genomics 10, 558–568.

    Article  PubMed  CAS  Google Scholar 

  • Maxam A. and Gilbert W. (1980) Sequencing end-labeled DNA with base-specific chemical cleavages. Methods in Enzymology 65, 499–560.

    Article  PubMed  CAS  Google Scholar 

  • Moazed D., Stern S., and Noller H. F. (1986) Rapid chemical probing of conformation in 16S ribosomal RNA and 30S ribosomal subunits using primer extension. J. Mol. Biol. 187, 399–416.

    Article  PubMed  CAS  Google Scholar 

  • Mueller P. R. and Wolf B. (1989) In vivo footprinting of a muscle-specific enhancer by ligation mediated PCR. Science 246, 780–786. (This laboratory also makes an updated printed protocol for in vivo footprinting available on request.)

    Article  PubMed  CAS  Google Scholar 

  • O/rsbrien T. and Lis J. T. (1991) RNA polymerase II pauses at the 5’ end of the transcriptionally induced Drosophila-hsp70 gene. Mol. Cell. Biol. 11, 5285–5290.

    Google Scholar 

  • Onteniente B., Horellou P., Makeh I., Lisovski E., Grimber G., Briand P., Mallet J., and Peschanski M A (1991) A c-fos-NGF transgenic mice. New perspectives in the study of the factor. Sot. Neurosci. Abstr. 17, Part 1, p. 1117.

    Google Scholar 

  • Ozcelik T., Rosenthal A., and Francke U. (1991) Chromosomal mapping of brain-derived neurotrophic factor and neurotrophin-3 genes in man and mouse. Genomics 10, 569–575.

    Article  PubMed  CAS  Google Scholar 

  • Ross J. and Kobs G. (1986) H4 histone messenger RNA decay in cell-free extracts initiates at or near the 3’ terminus and proceeds 3’ to 5’. J. Mol. Biol. 188, 579–593.

    Article  PubMed  CAS  Google Scholar 

  • Rossant J. (1990) Manipulating the mouse genome: implications for neuro-biology. Neuron 4, 323–334.

    Article  PubMed  CAS  Google Scholar 

  • Russo A. F., Crenshaw III E. B., Lira S. A., Simmons D. M., Swanson L. W., and Rosenfeld M. G. (1988) Neuronal expression of chuneric genes in transgenic mice. Neuron 1, 311–320.

    Article  PubMed  CAS  Google Scholar 

  • Saltzman A. G. and Wemmann R. (1989) Promoter specrfrcity and modulation of RNA polymerase II transcription. FASEB J. 3, 1723–1733.

    PubMed  CAS  Google Scholar 

  • Sambrook J., Fritsch E. F., and Maniatis T. (1989) Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.

    Google Scholar 

  • Sariola H., Saarma M., Sainio K., Arumae U., Palgi J., Vaahtokari A., Thesleff I., and Karavanov A. (1991) Dependence of kidney morphogenesis on the expression of nerve growth factor receptor. Science 254, 571–573.

    Article  PubMed  CAS  Google Scholar 

  • Selden R. F., Howie K. B., Rowe M. E., Goodman H. M., and Moore D. D. (1986) Human growth hormone as a reporter gene in regulation stud-ies employing transient gene expression. Mol. Cell. Biol. 6, 3173–3179.

    PubMed  CAS  Google Scholar 

  • Shaw G. and Kamen K. (1986) A conserved AU sequence from the 3’ untranslated region of GM-CSF mRNA mediates selective mRNA degradation. Cell 46, 659–667.

    Article  PubMed  CAS  Google Scholar 

  • Sternberg N. (1990) Bacteriophage Pl cloning system for the isolation, amplification, and recovery of DNA fragments as large as 100 kilobase pairs. Proc. Natl. Acud. Sei. USA 87, 103–107.

    Article  CAS  Google Scholar 

  • Ullrich A., Gray A., Berman C., and Dull T J. (1983) Human beta-nerve growth factor gene sequence highly homologous to that of mouse. Nature 303, 821–825.

    Article  PubMed  CAS  Google Scholar 

  • Wickens M. and Stephenson P. (1984) Role of the conserved AAUAAA sequence: four AAUAAA point mutations prevent messenger RNA 3’ end formation. Sctence 226, 1045–1051.

    Article  CAS  Google Scholar 

  • Yashpe J. and Halvorson H. 0. (1976) /gb-D-galactosidase activity m single yeast ceils during cell cycle in Saccharomyces lactis. Science 191, 1283.

    Article  Google Scholar 

  • Zheng M. and Heinrich G. (1988) Structural and functional analysis of the promoter region of the nerve growth factor gene. Mol. Brain Res. 3, 133–140.

    Article  CAS  Google Scholar 

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© 1993 Humana Press Inc.

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Heinrich, G., Martin, S. (1993). Molecular Analysis of Neurotrophin Genes. In: Boulton, A.A., Baker, G.B., Hefti, F. (eds) Neurotrophic Factors. Neuromethods, vol 25. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-249-3:107

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  • DOI: https://doi.org/10.1385/0-89603-249-3:107

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-0-89603-249-1

  • Online ISBN: 978-1-59259-630-0

  • eBook Packages: Springer Protocols

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