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M13 Phage Growth and DNA Purification Using 96 Well Microtiter Trays

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DNA Sequencing Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 23))

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Abstract

The growth and purification of M13 DNA from small volume (1.5mL) cultures is a rapid and easily performed procedure (1). The samples can be processed in microcentrifuges in disposable polypropylene tubes and yield sufficient, pure single-stranded DNA (4 μM) for five or more sequencing experiments. Even when several cultures are to be grown and purified simultaneously, up to 100 can be processed to completion in a day. The handling of this number of samples is tedious, however, and much time is spent opening and closing tubes and transferring tubes in and out of microcentrifuges. One hundred small volume phenol extractions and ethanol precipitations tasks even the more dedicated sequencer.

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References

  1. Messing, J and Bankier, A. T. (1989) The use of single-stranded DNA phage in DNA sequencing, in Nucleic Acids Sequencing. A Practical Approach (Howe, C. J. and Ward, E. S., eds ), IRL Press, Oxford, pp. 1–36

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Author information

Authors and Affiliations

  1. MRC Lab of Molecular Biology, Cambridge, England

    Alan T. Bankier

Authors
  1. Alan T. Bankier
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Editor information

Editors and Affiliations

  1. Institute of Food Research, Norwich Research Park, Norwich, England

    Hugh G. Griffin  & Annette M. Griffin  & 

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© 1993 Humana Press Inc. Totowa, New Jersey

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Bankier, A.T. (1993). M13 Phage Growth and DNA Purification Using 96 Well Microtiter Trays. In: Griffin, H.G., Griffin, A.M. (eds) DNA Sequencing Protocols. Methods in Molecular Biology™, vol 23. Humana Press. https://doi.org/10.1385/0-89603-248-5:41

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  • DOI: https://doi.org/10.1385/0-89603-248-5:41

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-248-4

  • Online ISBN: 978-1-59259-510-5

  • eBook Packages: Springer Protocols

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