Abstract
The bacteriophage M13 has been developed into a cloning vector system for obtaining single-stranded DNA template required for the dideoxy chain termination method of sequencing DNA (1,2). General aspects of bacteriophage M13 as a cloning vector system are reviewed in Chapter 2, and the preparation of foreign DNA fragments for M13 cloning is described in Chapter 7. In this chapter the preparation of M13 vectors and the ligation of foreign DNA fragments (inserts) into M13 vectors are described.
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References
Messing, J. (1983) New M13 vectors for cloning Meth Enzymol. 101, 20–79
Sanger, F., Nicklen, S, and Coulson, A. R. (1977) DNA sequencmg with chamterminating inhlbltors Proc. Nat1 Acad. Sci. USA 74, 5463–5467
Yamsch-Perron, C, Vlelra, J., and Messing, J (1985) Improved M13 phage cloning vectors and host strains: nucleotlde sequences of the M13mp18 and pUC19 vectors. Gene 33, 103–119.
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© 1993 Humana Press Inc. Totowa, New Jersey
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Yu, Q. (1993). Cloning into M13. In: Griffin, H.G., Griffin, A.M. (eds) DNA Sequencing Protocols. Methods in Molecular Biology™, vol 23. Humana Press. https://doi.org/10.1385/0-89603-248-5:23
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DOI: https://doi.org/10.1385/0-89603-248-5:23
Publisher Name: Humana Press
Print ISBN: 978-0-89603-248-4
Online ISBN: 978-1-59259-510-5
eBook Packages: Springer Protocols