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DNA Sequencing Protocols pp 183–190Cite as

Sequencing of Double-Stranded PCR Products

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 23))

Abstract

The polymerase chain reaction (PCR) is fast becoming a standard protocol in many laboratories for cloning and analysis of small quantities of DNA (1). It involves the exponential synthesis of linear DNA molecules from double-stranded DNA templates using specific oligonucleotide primers for the DNA synthesis reaction. Because of its unique sensitivity and general applicability, the PCR procedure has been used in a variety of systems.

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References

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Author information

Authors and Affiliations

  1. MSU-DOE Plant Research Lab, East Lansing, MI

    Susannah Gal

Authors
  1. Susannah Gal
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Editor information

Editors and Affiliations

  1. Institute of Food Research, Norwich Research Park, Norwich, England

    Hugh G. Griffin  & Annette M. Griffin  & 

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© 1993 Humana Press Inc. Totowa, New Jersey

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Cite this protocol

Gal, S. (1993). Sequencing of Double-Stranded PCR Products. In: Griffin, H.G., Griffin, A.M. (eds) DNA Sequencing Protocols. Methods in Molecular Biology™, vol 23. Humana Press. https://doi.org/10.1385/0-89603-248-5:183

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  • DOI: https://doi.org/10.1385/0-89603-248-5:183

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-248-4

  • Online ISBN: 978-1-59259-510-5

  • eBook Packages: Springer Protocols

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