Abstract
The cloning of eukaryotic DNA in bacteria or by the polymerase chain reaction (PCR) inevitably results in the loss of much information, Protein-DNA contacts and cytosine methylation patterns, both important in gene regulation and other cellular processes, are not reproduced in these cloning procedures. To retain information on cytosine methylation and protein footprints, the DNA has to be sequenced in its native genomic state. Direct sequencing of genomic DNA followed by detection of specific sequences is referred to here to as “Genomic Sequencing.”
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© 1993 Humana Press Inc. Totowa, New Jersey
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Pfeifer, G.P., Riggs, A.D. (1993). Genomic Sequencing. In: Griffin, H.G., Griffin, A.M. (eds) DNA Sequencing Protocols. Methods in Molecular Biology™, vol 23. Humana Press. https://doi.org/10.1385/0-89603-248-5:169
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DOI: https://doi.org/10.1385/0-89603-248-5:169
Publisher Name: Humana Press
Print ISBN: 978-0-89603-248-4
Online ISBN: 978-1-59259-510-5
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