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DNA Sequencing Protocols pp 121–130Cite as

Electrophoresis of Sequence Reaction Samples

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 23))

Abstract

The underlying principle of both main DNA sequencing methods by Sanger (1) and Maxam and Gilbert (2), is the ability to fractionate and resolve long, single-stranded DNAmolecules that differ in length by only one nucleotide. Denaturing polyacrylamide gels have been reported to give interpretable separation of molecules up to 0.6 kb in length, on l-m long gels (3).

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References

  1. Sanger, F, Nicklen, S, and Coulson, A R. (1977) DNA sequencmg wtth chamterminating inhibitors. Proc. Natl. Acad. Sci. USA, 74, 5463–5467.

    Article  PubMed  CAS  Google Scholar 

  2. Maxam, A. M. and Gilbert, W. (1977) A New Method for Sequencing DNA Proc. Natl. Acad. Sci USA 74, 560–564.

    Article  PubMed  CAS  Google Scholar 

  3. Ansorge, W. and Barker, R. (1984) System for DNA sequencing with resolution of up to 600 base pairs. J Biochem. Blophys. Meth. 9, 33–47.

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  4. Bankier, A. T. and Barrell, B. G. (1983) Shotgun DNA sequencing, in Techniques in Life Sciences, Nucleic Acid Biochemistry vol. B5 (Flavell, R. A, ed.), Elsevier Screntific Publishers, Ireland, pp. 1–34.

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  5. Biggin, M. D., Gibson, T. J., and Hong, G. F. (1983) Buffer gradient gels and 35S label as an aid to raptd DNA sequence determination Proc. Natl. Acad. Sci. USA 80, 3963–3965.

    Article  PubMed  CAS  Google Scholar 

  6. Bankier, A. T and Barrell, B. G. (1989) Sequencing smgle-stranded DNA using the chain termmation method, in Nucleic Acids Sequencing: A Practical Approach (Howe, C J and Ward, E. S., eds.), IRL Press, Oxford, pp. 37–38.

    Google Scholar 

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Author information

Authors and Affiliations

  1. MRC Lab of Molecular Biology, Cambridge, England

    Alan T. Bankier

Authors
  1. Alan T. Bankier
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Editor information

Editors and Affiliations

  1. Institute of Food Research, Norwich Research Park, Norwich, England

    Hugh G. Griffin  & Annette M. Griffin  & 

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© 1993 Humana Press Inc. Totowa, New Jersey

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Cite this protocol

Bankier, A.T. (1993). Electrophoresis of Sequence Reaction Samples. In: Griffin, H.G., Griffin, A.M. (eds) DNA Sequencing Protocols. Methods in Molecular Biology™, vol 23. Humana Press. https://doi.org/10.1385/0-89603-248-5:121

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  • DOI: https://doi.org/10.1385/0-89603-248-5:121

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-248-4

  • Online ISBN: 978-1-59259-510-5

  • eBook Packages: Springer Protocols

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