Skip to main content

MicroGenie: Protein Analysis

  • Protocol
Computer Analysis of Sequence Data

Part of the book series: Methods in Molecular Biology ((MIMB,volume 24))

  • 575 Accesses

Abstract

At an initial inspection, MicroGenie appears to have a limited range of procedures designed specifically to analyze proteins; however, many of its procedures can be used with both proteins and nucleic acids. By suitable selection of control parameters, the protein analysis procedures can be made to use a variety of analysis methods that would probably appear as separate menu items in other systems. The homology search and comparison procedures, under the DATA BANK and COMPARE menus, may be used with proteins or nucleic acids, and are covered in more detail in Chapter 19 (MicroGenie: Homology Searches). MicroGenie can calculate the sizes of proteins from their mobility on an electrophoresis gel in relation to known size markers, The MicroGenie manual gives details of this in the Files section, Reverse translation of protein to nucleic acids is discussed in Chapter 17 (MicroGenie: Translation).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Hopp, T. P. and Woods, K. R. (1981) Prediction of protein antigenic determinants from amino acid sequences. Proc. Natl. Acad. SCL USA 78, 3824–3828.

    Article  CAS  Google Scholar 

  2. Kyte, J. and Doolittle, R. F. (1982) A simple method for displaying the hydropathic character of a protein, J. Mol. Biol. 157, 105–132.

    Article  PubMed  CAS  Google Scholar 

  3. Parker, J. M. R., Guo, D., and Hodges, R. S. (1986) New hydrophilicity scale derived from high-performance liquid chromatography peptide retention data: Correlation of predicted surface residues with antigenicity and X-ray derived accessible sites. Biochemistry 25, 5425–5432.

    Article  PubMed  CAS  Google Scholar 

  4. Eisenberg, D., Schwarz, E., Komaromy, M., and Wall, R. (1984) Analysis of membrane and surface protein sequences with the hydrophobic moment plot. J. Mol. Biol. 179, 125–142.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1994 Humana Press Inc., Totowa, NJ

About this protocol

Cite this protocol

Merrifield, R.K. (1994). MicroGenie: Protein Analysis. In: Griffin, A.M., Griffin, H.G. (eds) Computer Analysis of Sequence Data. Methods in Molecular Biology, vol 24. Humana Press. https://doi.org/10.1385/0-89603-246-9:227

Download citation

  • DOI: https://doi.org/10.1385/0-89603-246-9:227

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-246-0

  • Online ISBN: 978-1-59259-511-2

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics