Abstract
The shotgun merge section of the MicroGenie program is used to combine a number of overlapping DNA sequences into a single, larger, contiguous sequence (contig). This is particularly useful for constructing the continuous sequence of a cDNA clone from its respective restriction fragments or for assembling the data generated by sequencing the exonuclease digestion subclones (deletion cloning) (1) of a cDNA clone.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Henikoff, S. (1984) Unidirectional digestion with exonuclease III creates targeted break points for DNA sequencing. Gene 28, 351–359.
Staden, R. ( 1987) Computer handling of DNA sequencing projects, m Nucleic Acid and Protein Sequence Analysis: A Practical Approach (Bishop, M. J. and Rawlings, C. J., eds.), IRL Press, Oxford, pp. 173–217.
Lopez, R., Kristensen, T, and Prydz, H. (1992) Database contamination Nature 355,211.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1994 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
Davies, P., Merrifield, R.K. (1994). MIicroGenie: Shotgun DNA Sequencing. In: Griffin, A.M., Griffin, H.G. (eds) Computer Analysis of Sequence Data. Methods in Molecular Biology, vol 24. Humana Press. https://doi.org/10.1385/0-89603-246-9:201
Download citation
DOI: https://doi.org/10.1385/0-89603-246-9:201
Publisher Name: Humana Press
Print ISBN: 978-0-89603-246-0
Online ISBN: 978-1-59259-511-2
eBook Packages: Springer Protocols