Abstract
DNA sequencing methodology was developed in the late 1970s (1,2) and has become one of the most widely used techniques in molecular biology. The amount of DNA sequencing performed worldwide has increased exponentially each year and this trend will almost certainly continue for the next few years at least. The importance of this technique is underlined by the volume of research funds now being channelled into genome sequencing projects, and by the large sums of money being invested in the development of automated sequencers and sequence analysis systems.
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References
Sanger, F., Nicklen, S., and Coulson, A. R. (1977) DNA sequencing with chainterminator mhibitors Proc. Natl. Acad. Sci. USA 74, 5463–5467
Maxam, A M. and Gilbert, W. (1977) A new method for sequencing DNA. Proc Natl. Acad. Sci USA 74, 560–564
Messing, J. and Bankier, A. T. (1989) The use of single-stranded DNA phage in DNA sequencing, in Nucleic Acids Sequencing A Practical Approach (Howe, C. J. and Ward, E. S., eds.), IRL Press, Oxford, UK, pp. 1–36
Anderson, S. (1981) Shotgun DNA sequencmg using cloned DNAse l-generated fragments. Nucleic Acids Res. 9, 3015.
Deininger, P L. (1983) Random subcloning of somcated DNA Appllcatlon to shotgun DNA sequence analysis. Anal Biocheni 129, 216
Bankrer, A. T., Weston, K. M., and Barrell, B. G. (1987) Random cloning and sequencing by the M13/dideoxynucleotide cham termination method, in Meth-ods in Enzymology, vol. 155 (Wu, R, ed.), Academtc, London, pp. 51–93.
Smithies, O., Engels, W. R., Devereux, J. R., Shghton, J. L, and Shen, S. (1981) Base substitutions, length differences and DNA strand asymmetries in the human G-gamma and A-gamma fetal globin gene region. Cell 26, 345–353.
Devereux, J., Haeberli, P., and Smithies, 0. (1984) A comprehensive set of sequence analysts programs for the VAX. Nucleic Acids Res. 12, 387–395.
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© 1994 Humana Press Inc., Totowa, NJ
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Griffin, H.G., Griffin, A.M. (1994). Computer Analysis of Sequence Data. In: Griffin, A.M., Griffin, H.G. (eds) Computer Analysis of Sequence Data. Methods in Molecular Biology, vol 24. Humana Press. https://doi.org/10.1385/0-89603-246-9:1
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DOI: https://doi.org/10.1385/0-89603-246-9:1
Publisher Name: Humana Press
Print ISBN: 978-0-89603-246-0
Online ISBN: 978-1-59259-511-2
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