Abstract
A transgenic animal represents an enormous investment in time and money. Animals can be destroyed through disease, fire, malfuncnons in the control of the environment, negligence, sabotage, or accidental disposal. Researchers can protect valuable transgenic lines from accrdental destruction by “banking” them in liquid nitrogen. Cryopreservation can also reduce animal costs by decreasing the number of live animals investigators must maintain. Often, when one is trying to produce a transgenic animal, some lines will be derived that may not initially appear interesting. These animals can be stored in liquid nitrogen for future recovery and study. The maintenance of just one line of mice, say 25 mice at 15 cents/d, can cost over $1000 (US) in a single year.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Pomeroy, K. O. (1991) Cryopreservation of transgenic mice. GATA 8, 95–101.
Kasai, M., Komi, J. H., Takakamo, A., Tsudura, H., Sakurai, T., and Machada, T. (1990) A simple method for mouse embryo cryopreservation in a low toxicuy vitrification solution, without appreciable loss of viability. J. Reprod. Fert. 89, 91–97.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1993 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
Pomeroy, K.O. (1993). Cryopreservation of Transgenic Mouse Lines. In: Murphy, D., Carter, D.A. (eds) Transgenesis Techniques. Methods in Molecular Biology™, vol 18. Humana Press. https://doi.org/10.1385/0-89603-245-0:239
Download citation
DOI: https://doi.org/10.1385/0-89603-245-0:239
Publisher Name: Humana Press
Print ISBN: 978-0-89603-245-3
Online ISBN: 978-1-59259-505-1
eBook Packages: Springer Protocols