Abstract
Operations, such as vasectomy (see Chapter 15) and oviduct transfer (see Chapter 20), are performed on anesthetized animals. It is essential that the experimenter is familiar with this procedure and that the anesthetic has been tested: A lethal dose of anesthetic administered prior to an oviduct transfer is an unfortunate end to a day spent collecting and microinjecting eggs. The anesthetic described here is Avertin (administered ip); other anesthetics, perhaps routinely used in the experimenter's animal house, may be used.
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© 1993 Humana Press Inc., Totowa, NJ
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Carter, D.A. (1993). Anesthetizing Mice. In: Murphy, D., Carter, D.A. (eds) Transgenesis Techniques. Methods in Molecular Biology™, vol 18. Humana Press. https://doi.org/10.1385/0-89603-245-0:135
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DOI: https://doi.org/10.1385/0-89603-245-0:135
Publisher Name: Humana Press
Print ISBN: 978-0-89603-245-3
Online ISBN: 978-1-59259-505-1
eBook Packages: Springer Protocols