Direct Radioactive Labeling of Polymerase Chain Reaction Products

  • Tim McDaniel
  • Stephen J. Meltzer
Part of the Methods in Molecular Biology book series (MIMB, volume 15)


Radioactively labeled polymerase chain reaction (PCR) products are being used in an increasing number of molecular biology research techniques. Among these are PCR-based polymorphism assays such as linkage analysis (1) and detecting allelic loss in cancer cells (2). Other uses of radioactive PCR include generating probes for Southern and Northern blotting (3) and screening for polymorphisms and point mutations by the single-strand conformation polymorphism (SSCP) technique (4).


Polymerase Chain Reaction Polymerase Chain Reaction Product Allelic Loss Radioactive Contamination Triethyl Ammonium 
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Copyright information

© Humana Press Inc., Totowa, NJ 1993

Authors and Affiliations

  • Tim McDaniel
    • 1
  • Stephen J. Meltzer
    • 1
  1. 1.University of Maryland HospitalBaltimore

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