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Direct Radioactive Labeling of Polymerase Chain Reaction Products

  • Tim McDaniel
  • Stephen J. Meltzer
Part of the Methods in Molecular Biology book series (MIMB, volume 15)

Abstract

Radioactively labeled polymerase chain reaction (PCR) products are being used in an increasing number of molecular biology research techniques. Among these are PCR-based polymorphism assays such as linkage analysis (1) and detecting allelic loss in cancer cells (2). Other uses of radioactive PCR include generating probes for Southern and Northern blotting (3) and screening for polymorphisms and point mutations by the single-strand conformation polymorphism (SSCP) technique (4).

Keywords

Polymerase Chain Reaction Polymerase Chain Reaction Product Allelic Loss Radioactive Contamination Triethyl Ammonium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Peterson, M. B., Economou, E. P., Slaugenhaupt, S. A., Chakravarti, A., and Antonarakis, S. E. (1990) Linkage analysis of the human HMG 14 gene on chromosome 21 using a GT dinucleotide repeat as polymorphic marker. Genomics 7, 136–138.CrossRefGoogle Scholar
  2. 2.
    Bookstein, R., Rio, P., Madreperla, S.A., Hong, F., Allred, C., Grizzle, W. E., and Lee, W.-H. (1990) Promoter deletion and loss of retinoblastoma gene expression in human prostate carcinoma. Proc. Nat. Acad. Sci. USA 87, 7762–7766.PubMedCrossRefGoogle Scholar
  3. 3.
    Schowalter, D. B. and Sommer, S. S. (1989) The generation of radiolabeled DNA and RNA probes with polymerase chain reaction. Anal. Biochem. 177, 90–94.PubMedCrossRefGoogle Scholar
  4. 4.
    Orita, M., Suzuki, Y., Sekiya, T., and Hayashi, K. (1989) Rapid and sensitive detection of point mutations and DNA polymorphisms using the polymerase chain reaction. Genomics 5, 874–879.PubMedCrossRefGoogle Scholar
  5. 5.
    Hayashi, S., Orita, M., Suzuki, Y., and Sekiya, T. (1989) Use of labeled primers in polymerase chain reaction (LP-PCR) for a rapid detection of the product. Nucleic Acids Res. 18, 3605.CrossRefGoogle Scholar
  6. 6.
    McDaniel, T. K., Huang, Y., Yin, J., Needleman, S. W., and Meltzer, S. J. (1991) Direct radiolabeling of unpurified PCR product using Klenow fragment. BioTechniques 11, 7,8.Google Scholar
  7. 7.
    O’Farrell, P. H., Kutter, E., and Nakanishi (1980) A restriction map of the bacteriophage T4 genome. Mol. Gen. Genet. 179, 421–435.PubMedCrossRefGoogle Scholar
  8. 8.
    Nelkin, B. D. (1990) Labeling of double stranded oligonucleotides to high specific activity. BioTechniques 8, 616–618.PubMedGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 1993

Authors and Affiliations

  • Tim McDaniel
    • 1
  • Stephen J. Meltzer
    • 1
  1. 1.University of Maryland HospitalBaltimore

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