Direct Radioactive Labeling of Polymerase Chain Reaction Products
Radioactively labeled polymerase chain reaction (PCR) products are being used in an increasing number of molecular biology research techniques. Among these are PCR-based polymorphism assays such as linkage analysis (1) and detecting allelic loss in cancer cells (2). Other uses of radioactive PCR include generating probes for Southern and Northern blotting (3) and screening for polymorphisms and point mutations by the single-strand conformation polymorphism (SSCP) technique (4).
KeywordsPolymerase Chain Reaction Polymerase Chain Reaction Product Allelic Loss Radioactive Contamination Triethyl Ammonium
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