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PCR Protocols pp 339–348Cite as

Single Specific Primer-Polymerase Chain Reaction (SSP-PCR) and Genome Walking

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 15))

Abstract

The polymerase chain reaction (PCR) is used for selective amplification of DNA fragments from both prokaryotes and eukaryotes (1–3). The only requirement for amplification is that the sequence of the extremities of the DNA fragment to be amplified be known (4). This places a limitation on the use of PCR in the amplification of adjacent unknown regions. We have developed a method that allows the amplification of double-stranded DNA even when the sequence information is available at one end only (5). This method, the single specific primer-PCR (SSP-PCR), permits amplification of genes for which only a partial sequence information is available, and allows unidirectional genome walking from known into unknown regions of the chromosome.

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Author information

Authors and Affiliations

  1. Chiron Corporation, Emeryville, CA

    Venkatakrishna Shyamala

  2. Department of Molecular and Cell Biology, University of California, Berkeley, CA

    Giovanna Ferro-Luzzi Ames

Authors
  1. Venkatakrishna Shyamala
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  2. Giovanna Ferro-Luzzi Ames
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Editor information

Editors and Affiliations

  1. University of Connecticut Health Center, Farmington, CT

    Bruce A. White

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© 1993 Humana Press Inc., Totowa, NJ

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Shyamala, V., Ferro-Luzzi Ames, G. (1993). Single Specific Primer-Polymerase Chain Reaction (SSP-PCR) and Genome Walking. In: White, B.A. (eds) PCR Protocols. Methods in Molecular Biology, vol 15. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-244-2:339

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  • DOI: https://doi.org/10.1385/0-89603-244-2:339

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-0-89603-244-6

  • Online ISBN: 978-1-59259-502-0

  • eBook Packages: Springer Protocols

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