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PCR Protocols pp 317-337 | Cite as

Use of Degenerate Oligonucleotide Primers and the Polymerase Chain Reaction to Clone Gene Family Members

  • Gregory M. Preston
Part of the Methods in Molecular Biology book series (MIMB, volume 15)

Abstract

As more and more genes are cloned and sequenced, it is apparent that nearly all genes are related to other genes. Similar genes are grouped into families. Examples of gene families include the collagen, globin, and myosin gene families. There are also gene superfamilies. Gene superfamilies are composed of genes that have areas of high homology and areas of high divergence. Examples of gene superfamilies include the oncogenes, homeotic genes, and a newly recognized gene superfamily of transmembrane proteins related to the lens fiber cells major intrinsic protein, or the MIP gene superfamily (1). In most cases, the different members of a gene family carry out related functions.

Keywords

Polymerase Chain Reaction Sodium Dodecyl Sulfate Polymerase Chain Reaction Reaction Degenerate Primer CHIP28 Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 1993

Authors and Affiliations

  • Gregory M. Preston
    • 1
  1. 1.Departments of Medicine and Cell Biology /AnatomyJohns Hopkins University School of MedicineBaltimore

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