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Nonradioactive Labeling of Polymerase Chain Reaction Products

  • Udo Reischl
  • Rüdiger Rüger
  • Christoph Kessler
Part of the Methods in Molecular Biology book series (MIMB, volume 15)

Abstract

Polymerase chain reaction (PCR) was originally introduced to amplify in vitro particular DNA sequences by the application of temperature cycles (1). In a modification, RNA molecules also may serve as templates by an additional reverse transcription step converting RNA in complementary DNA sequences (2).

Keywords

Maleate Buffer Polymerase Chain Reaction Thermal Cycler Label Polymerase Chain Reaction Product Efficient Polymerase Chain Reaction Amplification Renaturation Solution 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 1993

Authors and Affiliations

  • Udo Reischl
    • 1
  • Rüdiger Rüger
    • 1
  • Christoph Kessler
    • 1
  1. 1.Department of Molecular GeneticsBoehringer Mannheim GmbHPenzberg/Obb.Germany

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