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The Culture and Preparation of Gametocytes of Plasmodium falciparum for Immunochemical, Molecular, and Mosquito Infectivity Studies

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Protocols in Molecular Parasitology

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 21))

Abstract

Since the development of methods for in vitro cultivation of the asexual blood stages of P.faZciparum (1) and the production of mature gametocytes from such cultures capable of infecting mosquitoes (2), it has been possible to conduct a wide range of studies on the sexual stages of P. filciparum. These include studies on the infectivity of the gametocytes of this parasite to mosquitoes (3) and the biology of the parasites in the vector (4) including studies on transmission blocking immunity using monoclonal antibodies against gamete surface antigens (5) and human sera following natural malarial infections (6). The developmental origin of sexual stages of P. fulcipurum has been studied in culture (7) and biochemical and immunochemical studies have been done to identify and characterize sexual stage specific proteins, especially those that are targets of transmission blocking antibodies (5). The genes encoding several sexual stage specific proteins have been identified and fully sequenced (8–10).

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Author information

Authors and Affiliations

  1. Institute of Cell, Animal, and population Biology, University of Edinburgh, Scotland, UK

    Richard Carter & Lisa Ranford-Cartwright

  2. Laboratorio di Biologia Cellulare, Istituto Superiore di Sanita, Rome, Italy

    Pietro Alano

Authors
  1. Richard Carter
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  2. Lisa Ranford-Cartwright
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  3. Pietro Alano
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Editor information

Editors and Affiliations

  1. University of Manchester Institute of Science and Technology, Manchester, UK

    John E. Hyde

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© 1993 Humana Press Inc, Totowa, NJ

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Carter, R., Ranford-Cartwright, L., Alano, P. (1993). The Culture and Preparation of Gametocytes of Plasmodium falciparum for Immunochemical, Molecular, and Mosquito Infectivity Studies. In: Hyde, J.E. (eds) Protocols in Molecular Parasitology. Methods in Molecular Biology™, vol 21. Humana Press. https://doi.org/10.1385/0-89603-239-6:67

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  • DOI: https://doi.org/10.1385/0-89603-239-6:67

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-239-2

  • Online ISBN: 978-1-59259-508-2

  • eBook Packages: Springer Protocols

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