Abstract
Immunofluorescence, as the name suggests, involves the visualization of proteins and structures within cells using antibodies as fluorescent probes. It has proven to be an extremely valuable technique for several reasons:
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1.
Vast numbers of cells can be processed and observed in a single experiment;
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2.
Immunofluorescence facilitates the observation of individual cells and differences among them; and
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3.
Information is not only provided on the presence or absence of a protein, but also concerning its precise location within each cell.
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Further Reading
Hudson, L. and Hay, F. C. (1989) PracticalImmunology. Blackwell Scientific, Oxford, UK.
References
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Read, M., Sherwin, T., Holloway, S. P., Gull, K., and Hyde, J. E. (1993) Microtubular organization visualized by immunofluorescence microscopy during erythocytic schizogomy in Plasmodium falciparum and investigation of post-translational modifications of parasite tubulin. Parasitology 106, 223–232.
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© 1993 Humana Press Inc, Totowa, NJ
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Sherwin, T., Read, M. (1993). Immunofluorescence of Parasites. In: Hyde, J.E. (eds) Protocols in Molecular Parasitology. Methods in Molecular Biology™, vol 21. Humana Press. https://doi.org/10.1385/0-89603-239-6:407
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DOI: https://doi.org/10.1385/0-89603-239-6:407
Publisher Name: Humana Press
Print ISBN: 978-0-89603-239-2
Online ISBN: 978-1-59259-508-2
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