Immunofluorescence of Parasites

  • Trevor Sherwin
  • Martin Read
Part of the Methods in Molecular Biology™ book series (MIMB, volume 21)

Abstract

Immunofluorescence, as the name suggests, involves the visualization of proteins and structures within cells using antibodies as fluorescent probes. It has proven to be an extremely valuable technique for several reasons:
  1. 1.

    Vast numbers of cells can be processed and observed in a single experiment;

     
  2. 2.

    Immunofluorescence facilitates the observation of individual cells and differences among them; and

     
  3. 3.

    Information is not only provided on the presence or absence of a protein, but also concerning its precise location within each cell.

     

Keywords

Dust Mercury Malaria Fluorescein DAPI 

Further Reading

  1. Hudson, L. and Hay, F. C. (1989) PracticalImmunology. Blackwell Scientific, Oxford, UK.Google Scholar

References

  1. 1.
    Robinson, D. R. and Gull, K. (1991) Basal body movements as a mechanism for mitochondrial genome segregation in the trypanosome cell cycle. Nature 352, 731–733.PubMedCrossRefGoogle Scholar
  2. 2.
    Sasse, R. and Gull, K. (1988) Tubulin post-translational modifications and the construction of microtubular organelles in Trypanosoma brucei. J. Cell Sci. 90, 577–589.PubMedGoogle Scholar
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    Sherwin, T., Schneider, A., Sasse, R., Seebeck, T., and Gull, K. (1987) Distinct localisation and cell cycle dependence of COOH terminally tyrosinolated α-tubulin in the microtubules of Trypanosoma brucei. J. Cell Biol. 104, 439–446.PubMedCrossRefGoogle Scholar
  5. 5.
    Read, M., Sherwin, T., Holloway, S. P., Gull, K., and Hyde, J. E. (1993) Microtubular organization visualized by immunofluorescence microscopy during erythocytic schizogomy in Plasmodium falciparum and investigation of post-translational modifications of parasite tubulin. Parasitology 106, 223–232.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc, Totowa, NJ 1993

Authors and Affiliations

  • Trevor Sherwin
    • 1
  • Martin Read
    • 2
  1. 1.Department of Biochemistry and Molecular BiologyUniversity of ManchesterManchesterUK
  2. 2.Department of Biochemistry and Applied Molecular BiologyUniversity of Manchester Institute of Technology (UMIST)ManchesterUK

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