Abstract
Receptor-mediated endocytosis is an important mechanism by which the cell is able to take in macromolecules from the external environment and carry them through the necessary metabolic processes. Studies of the sequential steps of these events require methods of following the fate of the molecule from its internalization to its degradation in lysosomes or its transfer to other intracellular compartments.
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Ashwell, G. and Harford, J. (1982) Carbohydrate specific receptors of the liver. Ann. Rev. Biochem. 51, 531–554.
Harford, J. and Ashwell, G. (1982) in The Glycoconjugates, vol. 4 (Horowitz, M. I., ed.), Academic, New York, pp. 27–55.
Harford, J., Wolkoff, A. W., Ashwell, G., and Klausner, R. D. (1983) Monensin inhibits intracellular dissociation of asialoglycoprotein from its receptor. J. Cell Biol. 96, 1824–1828.
Kindberg, G. M., Ford, T. C., Rickwood, D., and Berg, T. (1984) Separation of endocytic vesicles in Nycodenz gradients. Anal. Biochem. 142, 455–462.
Berg, T., Kindberg, G. M., Ford, T. C., and Blotnhoff, R. (1985) Intracellular transport of asialoglycoproteins in rat hepatocytes-evidence for two sub-populations. Exp. Cell Res. 161, 285–296.
Ford, T. C. and Rickwood, D. (1982) Formation of isotonic Nycodenz gradients for cell separations. Anal. Biochem. 124, 293–398.
Tolleshaug, H. (1981) Binding and internalization of asialoglycoprotein by isolated rat-liver hepatocytes. Int. J. Biochem. 13,45–51.
Pittman, R. C., Green, S. R., Attie, A. D., and Steinberg, D. (1979) A radio-iodinated intracellularly-trapped ligand for determining the sites of plasma protein degradation in vivo. J. Biol. Chem. 254, 6876–6879.
Seglen, P. O. (1979) Separation approaches for liver and other cell sources, in Cell Populations, Methodological Surveys (B), Biochemistry, vol. 9 (Reid, E., ed.), Ellis Horwood, Chichester, England, pp. 25–46.
Berg, T. and Blomhoff, R. (1983) Fractionation of mammalian cells, in lodinated Density Gradient Media (Rickwood, D., ed.), IRL at Oxford University Press, Oxford, UK, pp. 173,174.
Rickwood, D. (1983) Properties of iodinated density gradient media, in lodinated Density Gradient Media (Rickwood, D., ed.), IRL at Oxford University Press, Oxford, UK, pp. 1–21.
Ford, T. C. and Graham, J. M. (1991) Formation of density gradients, in An Introduction to Centrifugation, Bios Scientific, Oxford, UK, pp. 39–46.
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Ford, T.C. (1993). The Analysis of Receptor-Mediated Endocytosis by Centrifugation in Isoosmotic Gradients. In: Graham, J.M., Higgins, J.A. (eds) Biomembrane Protocols. Methods in Molecular Biology, vol 19. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-236-1:71
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DOI: https://doi.org/10.1385/0-89603-236-1:71
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-0-89603-236-1
Online ISBN: 978-1-59259-506-8
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