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Biomembrane Protocols pp 253–267Cite as

Oligosaccharide Mapping and Sequence Analysis of Glycosaminoglycans

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 19))

Abstract

A strategy for determining the structure of a glycosaminoglycan (GAG) can be divided into three areas. The first is to establish its saccharide composition. This requires complete depolymerization of the parent chains to disaccharide products that can be purified, separated, and identified either by comparison with reference standards or by further structural analysis (1–6).

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Author information

Authors and Affiliations

  1. Department of Clinical Research and Cancer Research Campaign, Department of Medical Oncology, University of Manchester, Christie Hospital and Holt Radium Institute, Manchester, UK

    Jeremy E. Turnbull

Authors
  1. Jeremy E. Turnbull
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Editor information

Editors and Affiliations

  1. Merseyside Innovation Centre, Liverpool, UK

    John M. Graham

  2. Department of Molecular Biology and Biotechnology, University of Sheffield, UK

    Joan A. Higgins

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© 1993 Humana Press Inc.

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Turnbull, J.E. (1993). Oligosaccharide Mapping and Sequence Analysis of Glycosaminoglycans. In: Graham, J.M., Higgins, J.A. (eds) Biomembrane Protocols. Methods in Molecular Biology, vol 19. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-236-1:253

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  • DOI: https://doi.org/10.1385/0-89603-236-1:253

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-0-89603-236-1

  • Online ISBN: 978-1-59259-506-8

  • eBook Packages: Springer Protocols

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