Abstract
The choice of a protein assay for membranes and membrane proteins is dependent on a number of considerations: sensitivity, specificity (both with respect to variation between proteins and interference by nonprotein components), and simplicity.
Keywords
- Ethanesulfonic Acid
- Colored Protein
- Sodium Dodecyl Sulfate Solution
- Propanesulfonic Acid
- Sodium Tartrate
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References
Peterson, G. L. (1983) Determination of total protein. Methods Enzymol. 91, 95–119.
Rodriguez-Vico, F., Martinez-Cayuela, M., Garcia-Peregrin, E., and Ramirez, H. (1989) A procedure for eliminating interferences in the Lowry method of protein determination. Anal. Biochem. 183, 275–278.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265–275.
Smith, P. K., Krohn, R. I., Hermanson, G. T., Mallia, A. K., Gartner, F. H., Provenzano, M. D., Fujimoto, E. K., Goeke, N. M., Olson, B. J., and Klenk, D. C. (1985) Measurement of protein using bicinchoninic acid. Anal. Biochem. 150, 76–85.
Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248–254.
Peters, W. H. M., Fleuren-Jakobs, A. M. M., Kamps, K. M. P., de Pont, J. J. H. H. M., and Bonting, S. L. (1982) Lowry protein determination on membrane preparations: Need for standardization by amino acid analysis. Anal. Biochem. 124, 349–352.
Markwell, M. A. K., Haas, S. M., Bieber, L. L., and Tolbert, N. E. (1978) A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal. Biochem. 87, 206–210.
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© 1993 Humana Press Inc.
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Winterbourne, D.J. (1993). Chemical Assays for Proteins. In: Graham, J.M., Higgins, J.A. (eds) Biomembrane Protocols. Methods in Molecular Biology, vol 19. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-236-1:197
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DOI: https://doi.org/10.1385/0-89603-236-1:197
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-0-89603-236-1
Online ISBN: 978-1-59259-506-8
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