Abstract
Cholesterol is an amphipathic molecule of great importance in biology. It can be measured by enzymic and chemical methods, directly or indirectly following extraction from tissues or membranes. Before determination, cholesterol esters must be hydrolyzed using either chemical methods or cholesterol ester hydrolase (EC 3.11.13) (see Note 1). Enzymic methods for cholesterol determination are more sensitive and convenient and are generally used in preference to chemical methods.
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References
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© 1993 Humana Press Inc.
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Ahmed, H.A. (1993). Measurement of Cholesterol in Membranes. In: Graham, J.M., Higgins, J.A. (eds) Biomembrane Protocols. Methods in Molecular Biology, vol 19. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-236-1:179
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DOI: https://doi.org/10.1385/0-89603-236-1:179
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-0-89603-236-1
Online ISBN: 978-1-59259-506-8
eBook Packages: Springer Protocols