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The Isolation of Clathrin-Coated Vesicles and Purification of Their Protein Components

  • Antony P. Jackson
Part of the Methods in Molecular Biology book series (MIMB, volume 19)

Abstract

Coated vesicles are implicated in a number of receptor-mediated transport events within eukaryotic cells. In particular, they are required for the export of newly synthesized lysosomal enzymes from the trans-Golgi network, and at the plasma membrane they are responsible for receptor-mediated endocytosis of selected ligands (1). Coated vesicles are the best characterized of all transport organelles, owing in large measure to the ease with which they can be purified.

Keywords

Fraction Collector Isolation Buffer Coated Vesicle Dounce Homogenizer Sodium Thiocyanate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 1993

Authors and Affiliations

  • Antony P. Jackson
    • 1
  1. 1.Department of BiochemistryUniversity of CambridgeUK

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