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Polynucleotide Kinase (EC 2.7.1.78)

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Enzymes of Molecular Biology

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 16))

Abstract

The enzyme polynucleotide kinase (PNK, or ATP:5′-dephospho-polynucleotide 5′-phosphatase) catalyzes the transfer of a γ-phosphate group from a 5′-nucleoside triphosphate moiety to a free 5′-hydroxyl of a polynucleotide such as DNA or RNA, to form a 5′-phosphorylated DNA or RNA molecule and a nucleoside diphosphate.

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Authors and Affiliations

  1. Advanced Technologies (Cambridge) Ltd., Cambridge, UK

    Martin J. Maunders

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  1. Martin J. Maunders
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Editors and Affiliations

  1. Advanced Technologies (Cambridge) Limited, Cambridge, UK

    Michael M. Burrell

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© 1993 Humana Press Inc.

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Maunders, M.J. (1993). Polynucleotide Kinase (EC 2.7.1.78). In: Burrell, M.M. (eds) Enzymes of Molecular Biology. Methods in Molecular Biology™, vol 16. Humana Press. https://doi.org/10.1385/0-89603-234-5:343

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  • DOI: https://doi.org/10.1385/0-89603-234-5:343

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-322-1

  • Online ISBN: 978-1-59259-503-7

  • eBook Packages: Springer Protocols

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