DNA Methyltransferases (EC and EC

  • David P. Hornby
Part of the Methods in Molecular Biology™ book series (MIMB, volume 16)


DNA methyltransferases (Mtases) catalyze the transfer of the S-methyl group of S-adenosylmethionine (SAM) to deoxycytosine (dC) or deoxyadenine (dA) bases within defined DNA sequences (1). Individual enzymes are specific for one or the other base, and modify at the 6-NH2 of dA (EC or at the N4 or 5-C position of dC (EC depending on the particular enzyme (2). The reaction is predominantly irreversible. Enzymes, such as O 6-methylguanine DNA Mtase, that participate in DNA repair processes will not be discussed here.


Oligonucleotide Duplex MspI Site Restriction Enzyme Buffer Mouse Erythroleukemia Cell Optimal Ionic Strength 
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Copyright information

© Humana Press Inc. 1993

Authors and Affiliations

  • David P. Hornby
    • 1
  1. 1.Krebs InstituteSheffield UniversitySheffieldUK

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