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DNA Methyltransferases (EC 2.1.1.72 and EC 2.1.1.73)

  • David P. Hornby
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 16)

Abstract

DNA methyltransferases (Mtases) catalyze the transfer of the S-methyl group of S-adenosylmethionine (SAM) to deoxycytosine (dC) or deoxyadenine (dA) bases within defined DNA sequences (1). Individual enzymes are specific for one or the other base, and modify at the 6-NH2 of dA (EC 2.1.1.72) or at the N4 or 5-C position of dC (EC 2.1.1.73) depending on the particular enzyme (2). The reaction is predominantly irreversible. Enzymes, such as O 6-methylguanine DNA Mtase, that participate in DNA repair processes will not be discussed here.

Keywords

Oligonucleotide Duplex MspI Site Restriction Enzyme Buffer Mouse Erythroleukemia Cell Optimal Ionic Strength 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 1993

Authors and Affiliations

  • David P. Hornby
    • 1
  1. 1.Krebs InstituteSheffield UniversitySheffieldUK

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