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DNA Polymerases (EC 2.7.7.7)

  • Martin J. Maunders
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 16)

Abstract

DNA polymerases catalyze the synthesis of DNA molecules from monomeric deoxynucleotide triphosphate units. This definition encompasses those enzymes classed as DNA-dependent DNA polymerases (EC 2.7.7.7, which require both a DNA template strand and a DNA primer to which the monomeric units can be added), reverse transcriptases (EC 2.7.7.49, RNA-dependent DNA polymerases that utilize an RNA template), and terminal deoxynucleotidyl transferases (EC 2.7.7.31, which require no template). This chapter will consider only DNA-dependent DNA polymerases (EC 2.7.7.7, DNA nucleotidyltransferases, DNA-directed).

Keywords

Polymerase Activity Nick Translation Exonuclease Activity Klenow Fragment Label Nucleotide 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 1993

Authors and Affiliations

  • Martin J. Maunders
    • 1
  1. 1.Advanced Technologies (Cambridge) Ltd.CambridgeUK

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