Abstract
I describe herein a method in which pretreatment with low concentrations of uranyl acetate is used to increase the structural rigidity of protein-nucleic acid complexes, thereby substantially enhancing the information content of images obtained by high-resolution shadow-casting with tungsten, The visualization of three-dimensional objects by heavy-metal shadowcasting is along-established technique in electron microscopy (1), and it has long been realized that the highest resolution could be achieved by using carbon-metal mixtures or high-melting-point metals for the evaporation (2,3). Double-stranded DNAs, 2 nm in diameter, are readily visualized by high-resolution shadowing with tungsten (4).
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© 1994 Humana Press Inc, Totowa, NJ
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Gray, C.W. (1994). Electron Microscopy of Protein-Nucleic Acid Complexes. In: Jones, C., Mulloy, B., Thomas, A.H. (eds) Microscopy, Optical Spectroscopy, and Macroscopic Techniques. Methods in Molecular Biology, vol 22. Humana Press. https://doi.org/10.1385/0-89603-232-9:1
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DOI: https://doi.org/10.1385/0-89603-232-9:1
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