Abstract
Yeast artificial chromosomes (YACs; 1) are rapidly becoming the major cloning system to study eukaryotic genomes by physical mapping and chromosome walking projects (1–6). The main advantages of YACs over prokaryotic-based cloning systems are their large insert capacity (100–1500 kb), and their ability to maintain sequences that are unstable or not well represented in bacteriophage or cosmid genomic libraries.
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© 1992 The Humana Press Inc.
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Monaco, A.P., Lark, Z., Lehrach, H. (1992). Construction of Yeast Artificial Chromosome Libraries by Pulsed-Field Gel Electrophoresis. In: Burmeister, M., Ulanovsky, L. (eds) Pulsed-Field Gel Electrophoresis. Methods in Molecular Biology™, vol 12. Humana Press. https://doi.org/10.1385/0-89603-229-9:225
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DOI: https://doi.org/10.1385/0-89603-229-9:225
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