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Separation and Size Determination of DNA over a 10–,200 kbp Range

  • Craig A. Cooney
Part of the Methods in Molecular Biology™ book series (MIMB, volume 12)

Abstract

Some types of pulsed-field gel electrophoresis (PFGE), including CHEF (contour-clamped homogeneous electric field), give straight lanes and excellent separations of a wide range of DNA sizes on one gel (ref. 1 and  Chapters 1, 2, 5 7). Here conditions are described for the separation of DNA in the l0–,200-kbp size range by CHEF. CHEF resolution in this range exceeds that of many commonly used size standards. Production and use of a size standard giving a 10-kbp ladder in the l0–,200-kbp range (2) is described and examples of separations are shown.

Keywords

Pulse Time Partial Digest Electrophoresis Chamber Agarose Block Short Pulse Time 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

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Copyright information

© The Humana Press Inc. 1992

Authors and Affiliations

  • Craig A. Cooney
    • 1
  1. 1.Biology DepartmentBeckman Research Institute, City of HopeDuarte

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