Separation and Size Determination of DNA over a 10–,200 kbp Range

  • Craig A. Cooney
Part of the Methods in Molecular Biology™ book series (MIMB, volume 12)


Some types of pulsed-field gel electrophoresis (PFGE), including CHEF (contour-clamped homogeneous electric field), give straight lanes and excellent separations of a wide range of DNA sizes on one gel (ref. 1 and  Chapters 1, 2, 5 7). Here conditions are described for the separation of DNA in the l0–,200-kbp size range by CHEF. CHEF resolution in this range exceeds that of many commonly used size standards. Production and use of a size standard giving a 10-kbp ladder in the l0–,200-kbp range (2) is described and examples of separations are shown.


Pulse Time Partial Digest Electrophoresis Chamber Agarose Block Short Pulse Time 
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Copyright information

© The Humana Press Inc. 1992

Authors and Affiliations

  • Craig A. Cooney
    • 1
  1. 1.Biology DepartmentBeckman Research Institute, City of HopeDuarte

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