Encapsulation of Cells in Agarose Beads

  • Joan Overhauser
Part of the Methods in Molecular Biology™ book series (MIMB, volume 12)


For the analysis of large DNA fragments using pulsed-field gel electrophoresis (PFGE), it is necessary to first embed the cells in agarose to prevent shearing of the DNA during protein extraction and restriction enzyme digestion. The speed and efficiency with which these steps are performed is based on the diffusion rate of the buffers and proteins in each step.


Agarose Bead Yeast Chromosome Agarose Solution YEPD Medium Large Globule 
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  1. 1.
    Jackson, P. A. and Cook, P. R. (1985) A general method for preparing chromatin containing intact DNA. EMBO 4, 913–918.Google Scholar
  2. 2.
    Overhauser, J. and Radic, M. Z. (1987) Encapsulation of cells in agarose beads for use with pulsed-field gel electrophoresis. FOCUS 9, 8,9.Google Scholar

Copyright information

© The Humana Press Inc. 1992

Authors and Affiliations

  • Joan Overhauser
    • 1
  1. 1.Department of Biochemistry and Molecular BioogyThomas Jefferson UniversityPhiladelphla

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