Encapsulation of Cells in Agarose Beads
Part of the
Methods in Molecular Biology™
book series (MIMB, volume 12)
For the analysis of large DNA fragments using pulsed-field gel electrophoresis (PFGE), it is necessary to first embed the cells in agarose to prevent shearing of the DNA during protein extraction and restriction enzyme digestion. The speed and efficiency with which these steps are performed is based on the diffusion rate of the buffers and proteins in each step.
KeywordsAgarose Bead Yeast Chromosome Agarose Solution YEPD Medium Large Globule
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
Jackson, P. A. and Cook, P. R. (1985) A general method for preparing chromatin containing intact DNA. EMBO
, 913–918.Google Scholar
Overhauser, J. and Radic, M. Z. (1987) Encapsulation of cells in agarose beads for use with pulsed-field gel electrophoresis. FOCUS
, 8,9.Google Scholar
© The Humana Press Inc. 1992