Abstract
The composition of the oligosaccharide side chains of glycoproteins is often very complex. This demands analytical techniques with a high resolution. We have adapted the development of high-temperature capillary GC and GC-MS using thin-film thermostable bonded stationary phases for the analysis of large permethylated oligosaccharides up to about decasaccharides (1,2). The technique is suitable for the analysis of most of the O-linked type of oligosaccharides, but the N-linked type of oligosaccharides are usually too large.
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References
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© 1993 Humana Press Inc., Totowa, NJ
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Hansson, G.C., Karlsson, H. (1993). Gas Chromatography and Gas Chromatography-Mass Spectrometry of Glycoprotein Oligosaccharides. In: Hounsell, E.F. (eds) Glycoprotein Analysis in Biomedicine. Methods in Molecular Biology, vol 14. Humana Press. https://doi.org/10.1385/0-89603-226-4:47
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DOI: https://doi.org/10.1385/0-89603-226-4:47
Publisher Name: Humana Press
Print ISBN: 978-0-89603-226-2
Online ISBN: 978-1-59259-501-3
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