Abstract
Antibodies directed against mucin epitopes have a wide number of uses, including the construction of immunoassays for measuring secretion (1), preparing immunoprecipitates for measuring radioisotope incorporation (2), for use in tissue and cellular immunocytochemistry (1,3), for characterizing mucin subdomains (4), as markers of malignancy (5), and for the screening of cDNA expression libraries (6).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
McCool, D. J., Marcon, M. A, Forstner, J. F., and Forstner, G. G. (1990) The T-84 human colonic adenocarcinoma cell line produces mucin in culture and releases it in response to various secretogogues. Biochem. J. 267, 491–500.
Dekker, J., Van Beurden-Lamers, W. O., and Strous, G. J. (1989) Biosynthesis of gastric mucus glycoprotein of the rat. J. Biol. Chem. 264, 10,431–10,437.
Fahim, R. E. F., Specian, R. D., Forstner, G. G., and Forstner, J. F. (1987) Characterization and localization of the ‘putative’ link component in rat small intestinal mucin. Biochem.J. 243, 631–640.
Mantle, M., Forstner, G. G., and Forstner, J. F. (1984) Antigenic and structural features of goblet-cell mucin of human small intestine. Biochem.J. 217, 159–167.
Gendler, S., Taylor-Papadimitriou, J., Duhig, T., Rothbard, J., and Burchell, J. (1988) A highly immunogenic region of a human polymorphic epithelial mucin expressed by carcinomas is made up of tandem repeats. J. Biol. Chem. 263, 12,820–12,823.
Gum, J. R., Byrd, J. C., Hicks, J. W., Toribara, N. W., Lamport, T. A., and Kim, Y. S. (1989) Molecular cloning of human intestinal mucin cDNAs. J. Biol. Chem. 264, 6480–6487.
Forstner, G., Forstner, J., and Fahim, R. (1989) Small intestinal mucin: polymerization and the “Link Glycopeptide,” in Mucus and Related Topics (Chantler, E. and Ratcliffe, N. A., eds.), Society for Experimental Biology, The Company of Biologists, Ltd., Cambridge, UK, pp. 251–271.
Laemmli, U. K. (1970) Cleavage of structural proteins during assembly of the head of the bacteriophage T4. Nature (London) 227, 680–685.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265–275.
Redinbaugh, M. J. and Turley, R. B. (1986) Adaptation of the Bicinchoninic acid protein assay for use with microtiter plates and sucrose gradient fractions. Anal. Biochem. 153, 267–271.
Mantle, M. and Allen, A. (1978) A colorimetric assay for glycoproteins based on the periodic acid Schiff base stain. Biochem. Soc. Trans. 6, 607–609.
Gerken, T. A., Gupta, R., and Jentoft, N. (1992) A novel approach for chemically deglycosylating O-linked glycoproteins. The deglycosylation of sub-maxillary and respiratory mucins. Biochemistry 31, 639–648.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1993 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
Khatri, I., Forstner, G., Forstner, J. (1993). Preparation of Polyclonal Antibodies to Native and Modified Mucin Antigens. In: Hounsell, E.F. (eds) Glycoprotein Analysis in Biomedicine. Methods in Molecular Biology, vol 14. Humana Press. https://doi.org/10.1385/0-89603-226-4:225
Download citation
DOI: https://doi.org/10.1385/0-89603-226-4:225
Publisher Name: Humana Press
Print ISBN: 978-0-89603-226-2
Online ISBN: 978-1-59259-501-3
eBook Packages: Springer Protocols